Projects per year
Abstract
Cellular responses to environmental stress are frequently mediated by RNA-binding proteins(RBPs). Here, we examined global RBP dynamics in Saccharomyces cerevisiae in response to glucose starvation and heat shock. Each stress induced rapid remodeling of the RNA-protein interactome, without corresponding changes in RBP abundance. Consistent with general translation shutdown, ribosomal proteins contacting the mRNA showed decreased RNA association. Among translation components, RNA-association was most reduced for initiation factors involved in 40S scanning (eIF4A, eIF4B, and Ded1), indicating a common mechanism of translational repression. In unstressed cells, eIF4A, eIF4B, and Ded1 primarily targeted the 5′-ends of mRNAs. Following glucose withdrawal, 5’-binding was abolished within 30sec,explaining the rapid translation shutdown, but mRNAs remained stable. Heat shock induced progressive loss of 5’ RNA-binding by initiation factors over ~16min and provoked mRNA degradation, particularly for translation-related factors, mediated by Xrn1. Taken together, these results reveal mechanisms underlying translational control of gene expression during stress.
Original language | English |
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Pages (from-to) | 470-484.e8 |
Number of pages | 15 |
Journal | Molecular Cell |
Volume | 80 |
Issue number | 3 |
Early online date | 13 Oct 2020 |
DOIs | |
Publication status | Published - 5 Nov 2020 |
Keywords / Materials (for Non-textual outputs)
- protein-RNA interaction
- RNA binding sites
- UV crosslinking
- mass spectometry
- yeast
- stress responses
Fingerprint
Dive into the research topics of 'Stress-induced translation inhibition through rapid displacement of scanning initiation factors'. Together they form a unique fingerprint.Projects
- 5 Finished
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`Core Funding for the Wellcome Trust Centre for Cell Biology¿, Research Enrichment, Public Engagement
Tollervey, D. (Principal Investigator)
1/12/18 → 1/06/22
Project: Research
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Wellcome Centre for Cell Biology
Tollervey, D. (Principal Investigator)
1/12/16 → 1/12/21
Project: Research
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Proteomics at the Wellcome Trust Centre for Cell Biology (WTCCB) and School of Biological Sciences (SBS), Edinburgh
Rappsilber, J. (Principal Investigator)
1/10/15 → 30/09/20
Project: Research
Datasets
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Stress-induced translation inhibition through release of 40S scanning initiation factors
Bresson, S. (Creator) & Tollervey, D. (Creator), National Center for Biotechnology Information (Gene Expression Omnibus), 8 Jun 2020
https://www.ncbi.nlm.nih.gov/bioproject/?term=prjna623367
Dataset
Profiles
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David Tollervey
- School of Biological Sciences - Professor of RNA Biology
- Centre for Engineering Biology
Person: Academic: Research Active