Structural and Cellular Changes in Canine Myxomatous Mitral Valve Disease: An Image Analysis Study

R.I. Han, A. Black, G. Culshaw, A.T. French, B.M. Corcoran

Research output: Contribution to journalArticlepeer-review

Abstract

Background and aim of the study: Myxomatous mitral valve disease (MMVD) is the single most common cardiac disease of the dog, and bears close similarities to chronic degenerative mitral valve disease in humans. However, limited quantitative data are available on cellular and morphological changes in both species. The study aim was to use an image analysis system to examine various morphological changes associated with MMVD, and in particular to measure changes in cell numbers in overtly myxomatous areas of the distal portion of the valve. Methods: Mitral valve complexes were collected from normal dogs and dogs with varying severity of myxomatous mitral valve disease (veterinary Whitney grades 1-4; a measure of disease severity and age-related disease progression in the dog). An image analysis technique (ImageJ; National Institutes of Health, USA) was used to measure valve leaflet length, thickness, connective tissue content and density, glycosaminoglycan (GAG) content, cell number and shape in normal and myxomatous areas of diseased valves. Results: There was a change in the valve leaflet anterior/posterior length ratio in the diseased valves, suggestive of valve lengthening. Distinct and statistically significant (p
Original languageEnglish
Pages (from-to)60-70
Number of pages11
JournalJournal of Heart Valve Disease
Volume19
Issue number1
Publication statusPublished - Jan 2010

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