Structural and Functional Changes to Lymph Nodes in Ageing Mice

Vivian Turner; Neil Mabbott

doi:10.1111/imm.12727

Structural and Functional Changes to Lymph Nodes in Ageing Mice

Research output: Contribution to journal › Article › peer-review

Abstract

Lymph nodes (LN) are secondary lymphoid organs spread throughout the lymphatic system. They function to filter pathogenic material from the lymphatic fluid to maintain the health of the organism. Subcapsular sinus macrophages (SCSM) are amongst the first-responders within the LN due to their strategic location within the subcapsular sinus region. These macrophages aid the delivery of immune complexes to B cells and follicular dendritic cells (FDC) within the LN. Here we show an increase in SCSM and other macrophage populations within aged LN. However, immune complex uptake by macrophages within LN was not altered with age, nor was immune complex uptake by B cells. LN stromal cell populations, important in immune responses and the localisation and survival of leukocytes, were altered in their representation and distribution in aged LN. In particular FDC regions were decreased in size and had decreased chemokine CXCL13 expression. Furthermore, the retention of immune complexes by FDC was decreased in aged LN at 24 hours post- injection. Since FDC are important in the maintenance of germinal centre responses, the decreased retention of IC in aged LN may contribute to the reduced germinal centre responses observed in aged mice.

Original language	English
Journal	Immunology
Volume	151
Issue number	2
Early online date	16 Feb 2017
DOIs	https://doi.org/10.1111/imm.12727
Publication status	Published - Jun 2017

Keywords / Materials (for Non-textual outputs)

Lymph Node
Ageing
Follicular Dendritic Cells
Macrophages
Stromal cells

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10.1111/imm.12727

Turner_et_al-2017-Immunology (1)
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Final published version, 1.06 MBLicence: Creative Commons: Attribution (CC-BY)

Determining the effects of aging on the splenic marginal zone and the implications for host immunity
Mabbott, N. (Principal Investigator)
Biotechnology and Biological Sciences Research Council
1/03/14 → 28/02/17
Project: Research
Innate immunity and endemic diseases in livestock species
Collie, D. (Principal Investigator), Beard, P. (Co-investigator), Bishop, S. (Co-investigator), Bronsvoort, M. (Co-investigator), Burt, D. (Co-investigator), Fitzgerald, R. (Co-investigator), Freeman, T. (Co-investigator), Gally, D. (Co-investigator), Gill, A. (Co-investigator), Glass, E. (Co-investigator), Hocking, P. (Co-investigator), Hope, J. (Co-investigator), Hume, D. (Co-investigator), Kaiser, P. (Co-investigator), Mabbott, N. (Co-investigator), McLachlan, G. (Co-investigator), Morrison, L. (Co-investigator), Stevens, J. (Co-investigator), Stevens, M. (Co-investigator) & Watson, M. (Co-investigator)
Biotechnology and Biological Sciences Research Council
1/04/12 → 31/03/17
Project: Research

Cite this

@article{babecbcd03ef408aa6f380226af50c29,

title = "Structural and Functional Changes to Lymph Nodes in Ageing Mice",

abstract = "Lymph nodes (LN) are secondary lymphoid organs spread throughout the lymphatic system. They function to filter pathogenic material from the lymphatic fluid to maintain the health of the organism. Subcapsular sinus macrophages (SCSM) are amongst the first-responders within the LN due to their strategic location within the subcapsular sinus region. These macrophages aid the delivery of immune complexes to B cells and follicular dendritic cells (FDC) within the LN. Here we show an increase in SCSM and other macrophage populations within aged LN. However, immune complex uptake by macrophages within LN was not altered with age, nor was immune complex uptake by B cells. LN stromal cell populations, important in immune responses and the localisation and survival of leukocytes, were altered in their representation and distribution in aged LN. In particular FDC regions were decreased in size and had decreased chemokine CXCL13 expression. Furthermore, the retention of immune complexes by FDC was decreased in aged LN at 24 hours post- injection. Since FDC are important in the maintenance of germinal centre responses, the decreased retention of IC in aged LN may contribute to the reduced germinal centre responses observed in aged mice.",

keywords = "Lymph Node, Ageing, Follicular Dendritic Cells, Macrophages, Stromal cells",

author = "Vivian Turner and Neil Mabbott",

year = "2017",

month = jun,

doi = "10.1111/imm.12727",

language = "English",

volume = "151",

journal = "Immunology",

issn = "0019-2805",

publisher = "Wiley-Blackwell",

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TY - JOUR

T1 - Structural and Functional Changes to Lymph Nodes in Ageing Mice

AU - Turner, Vivian

AU - Mabbott, Neil

PY - 2017/6

Y1 - 2017/6

N2 - Lymph nodes (LN) are secondary lymphoid organs spread throughout the lymphatic system. They function to filter pathogenic material from the lymphatic fluid to maintain the health of the organism. Subcapsular sinus macrophages (SCSM) are amongst the first-responders within the LN due to their strategic location within the subcapsular sinus region. These macrophages aid the delivery of immune complexes to B cells and follicular dendritic cells (FDC) within the LN. Here we show an increase in SCSM and other macrophage populations within aged LN. However, immune complex uptake by macrophages within LN was not altered with age, nor was immune complex uptake by B cells. LN stromal cell populations, important in immune responses and the localisation and survival of leukocytes, were altered in their representation and distribution in aged LN. In particular FDC regions were decreased in size and had decreased chemokine CXCL13 expression. Furthermore, the retention of immune complexes by FDC was decreased in aged LN at 24 hours post- injection. Since FDC are important in the maintenance of germinal centre responses, the decreased retention of IC in aged LN may contribute to the reduced germinal centre responses observed in aged mice.

AB - Lymph nodes (LN) are secondary lymphoid organs spread throughout the lymphatic system. They function to filter pathogenic material from the lymphatic fluid to maintain the health of the organism. Subcapsular sinus macrophages (SCSM) are amongst the first-responders within the LN due to their strategic location within the subcapsular sinus region. These macrophages aid the delivery of immune complexes to B cells and follicular dendritic cells (FDC) within the LN. Here we show an increase in SCSM and other macrophage populations within aged LN. However, immune complex uptake by macrophages within LN was not altered with age, nor was immune complex uptake by B cells. LN stromal cell populations, important in immune responses and the localisation and survival of leukocytes, were altered in their representation and distribution in aged LN. In particular FDC regions were decreased in size and had decreased chemokine CXCL13 expression. Furthermore, the retention of immune complexes by FDC was decreased in aged LN at 24 hours post- injection. Since FDC are important in the maintenance of germinal centre responses, the decreased retention of IC in aged LN may contribute to the reduced germinal centre responses observed in aged mice.

KW - Lymph Node

KW - Ageing

KW - Follicular Dendritic Cells

KW - Macrophages

KW - Stromal cells

U2 - 10.1111/imm.12727

DO - 10.1111/imm.12727

M3 - Article

SN - 0019-2805

VL - 151

JO - Immunology

JF - Immunology

IS - 2

ER -

Structural and Functional Changes to Lymph Nodes in Ageing Mice

Abstract

Keywords / Materials (for Non-textual outputs)

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Projects

Determining the effects of aging on the splenic marginal zone and the implications for host immunity

Innate immunity and endemic diseases in livestock species

Cite this