Structure of the human VIPR2 gene for vasoactive intestinal peptide receptor type 2

E M Lutz, S Shen, M Mackay, K West, A J Harmar

Research output: Contribution to journalArticlepeer-review

Abstract

The VPAC(2) (vasoactive intestinal peptide (VIP)(2)) receptor is a seven-transmembrane spanning G protein-coupled receptor which responds similarly to VIP and pituitary adenylate cyclase activating polypeptide (PACAP) in stimulating cAMP production. Recently, we reported the localisation of the human VPAC(2) receptor gene (VIPR2) to chromosome 7q36.3 (Mackay, M. et al. (1996) Genomics 37, 345-353). Here, we describe the characterisation of the VIPR2 gene structure and promoter region. The VIPR2 gene is encoded by 13 exons, the initiator codon of the 438 amino acid open reading frame is located in exon 1 and the termination signal and a poly-adenylation signal sequence are located in exon 13. The 5' untranslated region extends 187 bp upstream of the initiator codon and is extremely GC-rich (80%), The poly-adenylation signal is located 2416 bp downstream of the stop codon, Intron sizes range from 68 hp (intron 11) to 45 kb (intron 4) and the human gene spans 117 kb. (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)197-203
Number of pages7
JournalFEBS Letters
Volume458
Issue number2
DOIs
Publication statusPublished - 17 Sep 1999

Keywords

  • 5' Untranslated Regions/genetics
  • Amino Acid Sequence
  • Base Sequence
  • Binding Sites/genetics
  • Chromosomes, Artificial, Yeast/genetics
  • Chromosomes, Human, Pair 7
  • Cloning, Molecular
  • Consensus Sequence
  • Cytosine
  • DNA, Complementary
  • Exons
  • GTP-Binding Proteins/metabolism
  • Guanine
  • Humans
  • Molecular Sequence Data
  • Promoter Regions, Genetic/genetics
  • Receptors, Vasoactive Intestinal Peptide
  • Transcription Factors

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