Study of DNA deformation under flow using optical tweezers

E Theofanidou, T J Su, J Arlt, D T F Dryden, W J Hossack, J Crain, Wilson Poon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract / Description of output

The stretching and unwinding of polymers under flow is important for understanding the rheological properties of dilute polymer solutions. Scaling theory based on the blob picture of single polymer chains predicts several regimes for the overall shape of a hydrodynamically deformed macromolecule. We studied the shape of a DNA molecule stretched out by steady uniform flow at different velocities using optical trapping of single DNA molecules (tethered on polystyrene beads) and single molecule fluorescence imaging. The results show a gradual transition from a non-draining regime at low velocities to a free-draining regime at high velocities, thus verifying the predictions of the free draining-shell (F-shell) blob model.

Original languageEnglish
Title of host publicationOPTICAL TRAPPING AND OPTICAL MICROMANIPULATION
EditorsK Dholakia, GC Spalding
Place of PublicationBELLINGHAM
PublisherSPIE
Pages712-723
Number of pages12
ISBN (Print)0-8194-5452-4
Publication statusPublished - 2004
EventConference on Optical Trapping and Optical Micromanipulation - Denver
Duration: 2 Aug 20046 Aug 2004

Conference

ConferenceConference on Optical Trapping and Optical Micromanipulation
CityDenver
Period2/08/046/08/04

Keywords / Materials (for Non-textual outputs)

  • DNA
  • optical tweezers
  • biophysics
  • polymer dynamics
  • fluid dynamics
  • STRETCHED POLYMER-CHAINS
  • TETHERED POLYMER
  • FLUORESCENCE MICROSCOPY
  • ELONGATIONAL FLOW
  • UNIFORM-FLOW
  • DYNAMICS
  • MOLECULES
  • MANIPULATION

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