Study of DNA deformation under flow using optical tweezers

E  Theofanidou; T J  Su; J  Arlt; D T F  Dryden; W J  Hossack; J  Crain; Wilson Poon

Study of DNA deformation under flow using optical tweezers

E Theofanidou, T J Su, J Arlt, D T F Dryden, W J Hossack, J Crain, Wilson Poon

School of Physics and Astronomy

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The stretching and unwinding of polymers under flow is important for understanding the rheological properties of dilute polymer solutions. Scaling theory based on the blob picture of single polymer chains predicts several regimes for the overall shape of a hydrodynamically deformed macromolecule. We studied the shape of a DNA molecule stretched out by steady uniform flow at different velocities using optical trapping of single DNA molecules (tethered on polystyrene beads) and single molecule fluorescence imaging. The results show a gradual transition from a non-draining regime at low velocities to a free-draining regime at high velocities, thus verifying the predictions of the free draining-shell (F-shell) blob model.

Original language	English
Title of host publication	OPTICAL TRAPPING AND OPTICAL MICROMANIPULATION
Editors	K Dholakia, GC Spalding
Place of Publication	BELLINGHAM
Publisher	SPIE
Pages	712-723
Number of pages	12
ISBN (Print)	0-8194-5452-4
Publication status	Published - 2004
Event	Conference on Optical Trapping and Optical Micromanipulation - Denver Duration: 2 Aug 2004 → 6 Aug 2004

Conference

Conference	Conference on Optical Trapping and Optical Micromanipulation
City	Denver
Period	2/08/04 → 6/08/04

Keywords / Materials (for Non-textual outputs)

DNA
optical tweezers
biophysics
polymer dynamics
fluid dynamics
STRETCHED POLYMER-CHAINS
TETHERED POLYMER
FLUORESCENCE MICROSCOPY
ELONGATIONAL FLOW
UNIFORM-FLOW
DYNAMICS
MOLECULES
MANIPULATION

Edinburgh Soft Matter and Statistical Physics Group: Programme Grant
Cates, M., Ackland, G., Egelhaaf, S., Evans, M., Poon, W. & Pusey, P.
EPSRC
1/10/03 → 31/03/08
Project: Research

Cite this

@inproceedings{64411e52056d4ef29c80b36c4c9fb473,

title = "Study of DNA deformation under flow using optical tweezers",

abstract = "The stretching and unwinding of polymers under flow is important for understanding the rheological properties of dilute polymer solutions. Scaling theory based on the blob picture of single polymer chains predicts several regimes for the overall shape of a hydrodynamically deformed macromolecule. We studied the shape of a DNA molecule stretched out by steady uniform flow at different velocities using optical trapping of single DNA molecules (tethered on polystyrene beads) and single molecule fluorescence imaging. The results show a gradual transition from a non-draining regime at low velocities to a free-draining regime at high velocities, thus verifying the predictions of the free draining-shell (F-shell) blob model.",

keywords = "DNA, optical tweezers, biophysics, polymer dynamics, fluid dynamics, STRETCHED POLYMER-CHAINS, TETHERED POLYMER, FLUORESCENCE MICROSCOPY, ELONGATIONAL FLOW, UNIFORM-FLOW, DYNAMICS, MOLECULES, MANIPULATION",

author = "E Theofanidou and Su, {T J} and J Arlt and Dryden, {D T F} and Hossack, {W J} and J Crain and Wilson Poon",

year = "2004",

language = "English",

isbn = "0-8194-5452-4",

pages = "712--723",

editor = "K Dholakia and GC Spalding",

booktitle = "OPTICAL TRAPPING AND OPTICAL MICROMANIPULATION",

publisher = "SPIE",

address = "United States",

note = "Conference on Optical Trapping and Optical Micromanipulation ; Conference date: 02-08-2004 Through 06-08-2004",

}

TY - GEN

T1 - Study of DNA deformation under flow using optical tweezers

AU - Theofanidou, E

AU - Su, T J

AU - Arlt, J

AU - Dryden, D T F

AU - Hossack, W J

AU - Crain, J

AU - Poon, Wilson

PY - 2004

Y1 - 2004

N2 - The stretching and unwinding of polymers under flow is important for understanding the rheological properties of dilute polymer solutions. Scaling theory based on the blob picture of single polymer chains predicts several regimes for the overall shape of a hydrodynamically deformed macromolecule. We studied the shape of a DNA molecule stretched out by steady uniform flow at different velocities using optical trapping of single DNA molecules (tethered on polystyrene beads) and single molecule fluorescence imaging. The results show a gradual transition from a non-draining regime at low velocities to a free-draining regime at high velocities, thus verifying the predictions of the free draining-shell (F-shell) blob model.

AB - The stretching and unwinding of polymers under flow is important for understanding the rheological properties of dilute polymer solutions. Scaling theory based on the blob picture of single polymer chains predicts several regimes for the overall shape of a hydrodynamically deformed macromolecule. We studied the shape of a DNA molecule stretched out by steady uniform flow at different velocities using optical trapping of single DNA molecules (tethered on polystyrene beads) and single molecule fluorescence imaging. The results show a gradual transition from a non-draining regime at low velocities to a free-draining regime at high velocities, thus verifying the predictions of the free draining-shell (F-shell) blob model.

KW - DNA

KW - optical tweezers

KW - biophysics

KW - polymer dynamics

KW - fluid dynamics

KW - STRETCHED POLYMER-CHAINS

KW - TETHERED POLYMER

KW - FLUORESCENCE MICROSCOPY

KW - ELONGATIONAL FLOW

KW - UNIFORM-FLOW

KW - DYNAMICS

KW - MOLECULES

KW - MANIPULATION

M3 - Conference contribution

SN - 0-8194-5452-4

SP - 712

EP - 723

BT - OPTICAL TRAPPING AND OPTICAL MICROMANIPULATION

A2 - Dholakia, K

A2 - Spalding, GC

PB - SPIE

CY - BELLINGHAM

T2 - Conference on Optical Trapping and Optical Micromanipulation

Y2 - 2 August 2004 through 6 August 2004

ER -

Study of DNA deformation under flow using optical tweezers

Abstract

Conference

Keywords / Materials (for Non-textual outputs)

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Projects

Edinburgh Soft Matter and Statistical Physics Group: Programme Grant

Cite this