Subset-specific expression of Toll-like receptors by bovine afferent lymph dendritic cells

Dirk Werling, Jayne Hope, Nazneen Siddiqui, Stephanie Widdison, Christopher Russell, Paul Sopp, Tracey J Coffey

Research output: Contribution to journalArticlepeer-review

Abstract

Within the ruminant system, several possibilities exist to generate dendritic cells migrating out from the tissue into the regional draining lymphnodes as afferent lymph dendritic cells (ALDC). Here, we analysed Toll-like receptor (TLR) 1–10 mRNA expression by using quantitative real-time PCR in highly purified subsets of bovine ALDC. As TLR expression may be influenced by pathogens or vaccines and their adjuvant, it is necessary to understand what TLRs are expressed in a steady-state system to elucidate specific differences and to potentially optimise targeted vaccines. In this study we have assessed the TLR expression profiles of the four main bovine ALDC subsets (cDC1 and cDC2-4). We demonstrate differences in TLR expression between the four subsets that may reflect the ability of these cells to respond to different pathogens or to respond to adjuvants.
Original languageEnglish
Article number44
JournalFrontiers in Veterinary Science
Volume4
Early online date14 Mar 2017
DOIs
Publication statusPublished - 3 Apr 2017
Externally publishedYes

Keywords

  • Toll like receptors
  • dendritic cell
  • afferent lymph
  • dendritic cell subsets

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