Substantial metabolic activity of human brown adipose tissue during warm conditions and cold-induced lipolysis of local triglycerides

Graeme Weir; Lynne E Ramage; Murat Akyol; Jonathan K Rhodes; Catriona J Kyle; Alison M Fletcher; Thomas H Craven; Sonia J Wakelin; Amanda J Drake; Maria-Lena Gregoriades; Ceri Ashton; Nick Weir; Edwin J R van Beek; Fredrik Karpe; Brian R Walker; Roland H Stimson

doi:10.1016/j.cmet.2018.04.020

Substantial metabolic activity of human brown adipose tissue during warm conditions and cold-induced lipolysis of local triglycerides

Graeme Weir, Lynne E Ramage, Murat Akyol, Jonathan K Rhodes, Catriona J Kyle, Alison M Fletcher, Thomas H Craven, Sonia J Wakelin, Amanda J Drake, Maria-Lena Gregoriades, Ceri Ashton, Nick Weir, Edwin J R van Beek, Fredrik Karpe, Brian R Walker, Roland H Stimson

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Current understanding of in vivo human brown adipose tissue (BAT) physiology is limited by a reliance on positron emission tomography (PET)/computed tomography (CT) scanning, which has measured exogenous glucose and fatty acid uptake but not quantified endogenous substrate utilization by BAT. Six lean, healthy men underwent ¹⁸fluorodeoxyglucose-PET/CT scanning to localize BAT so microdialysis catheters could be inserted in supraclavicular BAT under CT guidance and in abdominal subcutaneous white adipose tissue (WAT). Arterial and dialysate samples were collected during warm (∼25°C) and cold exposure (∼17°C), and blood flow was measured by ¹³³xenon washout. During warm conditions, there was increased glucose uptake and lactate release and decreased glycerol release by BAT compared with WAT. Cold exposure increased blood flow, glycerol release, and glucose and glutamate uptake only by BAT. This novel use of microdialysis reveals that human BAT is metabolically active during warm conditions. BAT activation substantially increases local lipolysis but also utilization of other substrates such as glutamate.

Original language	English
Pages (from-to)	1348-1355.e4
Number of pages	12
Journal	Cell Metabolism
Volume	27
Issue number	6
Early online date	24 May 2018
DOIs	https://doi.org/10.1016/j.cmet.2018.04.020
Publication status	Published - 5 Jun 2018

Keywords / Materials (for Non-textual outputs)

IN-VIVO
ENERGY-EXPENDITURE
SKELETAL-MUSCLE
ADULT HUMANS
FATTY-ACIDS
BLOOD-FLOW
THERMOGENESIS
ACTIVATION
MICRODIALYSIS
EXPOSURE

Access to Document

10.1016/j.cmet.2018.04.020

Substantial Metabolic Activity of Human BrownFinal published version, 2.07 MBLicence: Creative Commons: Attribution (CC-BY)

Tissue-specific control of cortisol versus corticosterone in humans
Walker, B.
UK-based charities
1/02/16 → 30/06/22
Project: Research
Regulation of brown adipose tissue activity in humans by glucocorticoids
Stimson, R.
MRC
1/03/13 → 28/02/18
Project: Research

Roland Stimson
- Roland.Stimsoned.acuk
- Deanery of Clinical Sciences - Personal Chair of Endocrinology
- Centre for Cardiovascular Science
- Edinburgh Imaging
Person: Academic: Research Active

Cite this

Weir, G., Ramage, L. E., Akyol, M., Rhodes, J. K., Kyle, C. J., Fletcher, A. M., Craven, T. H., Wakelin, S. J., Drake, A. J., Gregoriades, M.-L., Ashton, C., Weir, N., van Beek, E. J. R., Karpe, F., Walker, B. R., & Stimson, R. H. (2018). Substantial metabolic activity of human brown adipose tissue during warm conditions and cold-induced lipolysis of local triglycerides. Cell Metabolism, 27(6), 1348-1355.e4. https://doi.org/10.1016/j.cmet.2018.04.020

@article{593f535298cf44b3a04a6865c460e990,

title = "Substantial metabolic activity of human brown adipose tissue during warm conditions and cold-induced lipolysis of local triglycerides",

abstract = "Current understanding of in vivo human brown adipose tissue (BAT) physiology is limited by a reliance on positron emission tomography (PET)/computed tomography (CT) scanning, which has measured exogenous glucose and fatty acid uptake but not quantified endogenous substrate utilization by BAT. Six lean, healthy men underwent 18fluorodeoxyglucose-PET/CT scanning to localize BAT so microdialysis catheters could be inserted in supraclavicular BAT under CT guidance and in abdominal subcutaneous white adipose tissue (WAT). Arterial and dialysate samples were collected during warm (∼25°C) and cold exposure (∼17°C), and blood flow was measured by 133xenon washout. During warm conditions, there was increased glucose uptake and lactate release and decreased glycerol release by BAT compared with WAT. Cold exposure increased blood flow, glycerol release, and glucose and glutamate uptake only by BAT. This novel use of microdialysis reveals that human BAT is metabolically active during warm conditions. BAT activation substantially increases local lipolysis but also utilization of other substrates such as glutamate.",

keywords = "IN-VIVO, ENERGY-EXPENDITURE, SKELETAL-MUSCLE, ADULT HUMANS, FATTY-ACIDS, BLOOD-FLOW, THERMOGENESIS, ACTIVATION, MICRODIALYSIS, EXPOSURE",

author = "Graeme Weir and Ramage, {Lynne E} and Murat Akyol and Rhodes, {Jonathan K} and Kyle, {Catriona J} and Fletcher, {Alison M} and Craven, {Thomas H} and Wakelin, {Sonia J} and Drake, {Amanda J} and Maria-Lena Gregoriades and Ceri Ashton and Nick Weir and {van Beek}, {Edwin J R} and Fredrik Karpe and Walker, {Brian R} and Stimson, {Roland H}",

year = "2018",

month = jun,

day = "5",

doi = "10.1016/j.cmet.2018.04.020",

language = "English",

volume = "27",

pages = "1348--1355.e4",

journal = "Cell Metabolism",

issn = "1550-4131",

publisher = "Cell Press",

number = "6",

}

Weir, G, Ramage, LE, Akyol, M, Rhodes, JK, Kyle, CJ, Fletcher, AM, Craven, TH, Wakelin, SJ, Drake, AJ, Gregoriades, M-L, Ashton, C, Weir, N, van Beek, EJR, Karpe, F, Walker, BR & Stimson, RH 2018, 'Substantial metabolic activity of human brown adipose tissue during warm conditions and cold-induced lipolysis of local triglycerides', Cell Metabolism, vol. 27, no. 6, pp. 1348-1355.e4. https://doi.org/10.1016/j.cmet.2018.04.020

TY - JOUR

T1 - Substantial metabolic activity of human brown adipose tissue during warm conditions and cold-induced lipolysis of local triglycerides

AU - Weir, Graeme

AU - Ramage, Lynne E

AU - Akyol, Murat

AU - Rhodes, Jonathan K

AU - Kyle, Catriona J

AU - Fletcher, Alison M

AU - Craven, Thomas H

AU - Wakelin, Sonia J

AU - Drake, Amanda J

AU - Gregoriades, Maria-Lena

AU - Ashton, Ceri

AU - Weir, Nick

AU - van Beek, Edwin J R

AU - Karpe, Fredrik

AU - Walker, Brian R

AU - Stimson, Roland H

PY - 2018/6/5

Y1 - 2018/6/5

N2 - Current understanding of in vivo human brown adipose tissue (BAT) physiology is limited by a reliance on positron emission tomography (PET)/computed tomography (CT) scanning, which has measured exogenous glucose and fatty acid uptake but not quantified endogenous substrate utilization by BAT. Six lean, healthy men underwent 18fluorodeoxyglucose-PET/CT scanning to localize BAT so microdialysis catheters could be inserted in supraclavicular BAT under CT guidance and in abdominal subcutaneous white adipose tissue (WAT). Arterial and dialysate samples were collected during warm (∼25°C) and cold exposure (∼17°C), and blood flow was measured by 133xenon washout. During warm conditions, there was increased glucose uptake and lactate release and decreased glycerol release by BAT compared with WAT. Cold exposure increased blood flow, glycerol release, and glucose and glutamate uptake only by BAT. This novel use of microdialysis reveals that human BAT is metabolically active during warm conditions. BAT activation substantially increases local lipolysis but also utilization of other substrates such as glutamate.

AB - Current understanding of in vivo human brown adipose tissue (BAT) physiology is limited by a reliance on positron emission tomography (PET)/computed tomography (CT) scanning, which has measured exogenous glucose and fatty acid uptake but not quantified endogenous substrate utilization by BAT. Six lean, healthy men underwent 18fluorodeoxyglucose-PET/CT scanning to localize BAT so microdialysis catheters could be inserted in supraclavicular BAT under CT guidance and in abdominal subcutaneous white adipose tissue (WAT). Arterial and dialysate samples were collected during warm (∼25°C) and cold exposure (∼17°C), and blood flow was measured by 133xenon washout. During warm conditions, there was increased glucose uptake and lactate release and decreased glycerol release by BAT compared with WAT. Cold exposure increased blood flow, glycerol release, and glucose and glutamate uptake only by BAT. This novel use of microdialysis reveals that human BAT is metabolically active during warm conditions. BAT activation substantially increases local lipolysis but also utilization of other substrates such as glutamate.

KW - IN-VIVO

KW - ENERGY-EXPENDITURE

KW - SKELETAL-MUSCLE

KW - ADULT HUMANS

KW - FATTY-ACIDS

KW - BLOOD-FLOW

KW - THERMOGENESIS

KW - ACTIVATION

KW - MICRODIALYSIS

KW - EXPOSURE

U2 - 10.1016/j.cmet.2018.04.020

DO - 10.1016/j.cmet.2018.04.020

M3 - Article

C2 - 29805098

SN - 1550-4131

VL - 27

SP - 1348-1355.e4

JO - Cell Metabolism

JF - Cell Metabolism

IS - 6

ER -

Substantial metabolic activity of human brown adipose tissue during warm conditions and cold-induced lipolysis of local triglycerides

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

Access to Document

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Projects

Tissue-specific control of cortisol versus corticosterone in humans

Regulation of brown adipose tissue activity in humans by glucocorticoids

Profiles

Roland Stimson

Cite this