Projects per year
Abstract / Description of output
During nuclear surveillance in yeast, the RNA exosome functions together with the TRAMP
complexes. These include the DEAH-box RNA helicase Mtr4 together with an RNA-binding protein (Air1 or Air2) and a poly(A) polymerase (Trf4 or Trf5). To better determine how RNA substrates are targeted, we analyzed protein and RNA interactions for TRAMP components. Mass spectrometry identified three distinct TRAMP complexes formed in vivo. These complexes preferentially assemble on different classes of transcripts. Unexpectedly, on many substrates, including pre-rRNAs and pre-mRNAs, binding specificity is apparently conferred by Trf4 and Trf5. Clustering of mRNAs by TRAMP association shows co
enrichment for mRNAs with functionally related products, supporting the significance of surveillance in regulating gene expression. We compared binding sites of TRAMP components with multiple nuclear RNA binding proteins, revealing preferential colocalization of subsets of factors. TRF5 deletion reduces Mtr4 recruitment and increases RNA abundance for mRNAs specifically showing high Trf5 binding.
complexes. These include the DEAH-box RNA helicase Mtr4 together with an RNA-binding protein (Air1 or Air2) and a poly(A) polymerase (Trf4 or Trf5). To better determine how RNA substrates are targeted, we analyzed protein and RNA interactions for TRAMP components. Mass spectrometry identified three distinct TRAMP complexes formed in vivo. These complexes preferentially assemble on different classes of transcripts. Unexpectedly, on many substrates, including pre-rRNAs and pre-mRNAs, binding specificity is apparently conferred by Trf4 and Trf5. Clustering of mRNAs by TRAMP association shows co
enrichment for mRNAs with functionally related products, supporting the significance of surveillance in regulating gene expression. We compared binding sites of TRAMP components with multiple nuclear RNA binding proteins, revealing preferential colocalization of subsets of factors. TRF5 deletion reduces Mtr4 recruitment and increases RNA abundance for mRNAs specifically showing high Trf5 binding.
Original language | English |
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Article number | 3122 |
Journal | Nature |
Volume | 11 |
DOIs | |
Publication status | Published - 19 Jun 2020 |
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Dive into the research topics of 'Substrate specificity of the TRAMP nuclear surveillance complexes'. Together they form a unique fingerprint.Projects
- 4 Finished
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Protein structures in the context of time and space by mass spectrometry
1/06/14 → 31/05/21
Project: Research
Datasets
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Substrate specificity of the TRAMP nuclear surveillance complexes
Delan-Forino, C. (Creator), Spanos, C. (Creator), Rappsilber, J. (Creator) & Tollervey, D. (Creator), National Center for Biotechnology Information (Gene Expression Omnibus), 20 Apr 2020
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE135526
Dataset