Successful targeting of the mouse cystic fibrosis transmembrane conductance regulator gene in embryonal stem cells

J R Dorin, P Dickinson, E Emslie, A R Clarke, L Dobbie, M L Hooper, S Halford, B J Wainwright, D J Porteous

Research output: Contribution to journalArticlepeer-review

Abstract

We wish to construct a mouse model for the human inherited disease cystic fibrosis. We describe here the successful targeting in embryonal stem cells of the murine homologue (Cftr) of the cystic fibrosis transmembrane conductance regulator gene, as the first critical step towards this end. The targeting event precisely disrupts exon 10, the site of the major mutation in patients with cystic fibrosis. The targeted cells are pluripotent and competent to form chimaeras.
Original languageEnglish
Pages (from-to)101-5
Number of pages5
JournalTransgenic Research
Volume1
Issue number2
DOIs
Publication statusPublished - 1992

Keywords

  • Animals
  • Cystic Fibrosis
  • Cystic Fibrosis Transmembrane Conductance Regulator
  • Disease Models, Animal
  • Embryo, Mammalian
  • Exons
  • Humans
  • Membrane Proteins
  • Mice
  • Mice, Transgenic
  • Restriction Mapping
  • Stem Cells

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