SUMOylation stabilizes sister kinetochore biorientation to allow timely anaphase

Bessie Su, Menglu Wang, Claudia Schaffner, Olga O. Nerusheva, Dean Clift, Christos Spanos, David Kelly, Michael H Tatham, Andreas Wallek, Yehui Wu, Juri Rappsilber, Arockia A. Jeyaprakash, Zuzana Storchova, Ronald T Hay, Adele L Marston

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

During mitosis, sister chromatids attach to microtubules from opposite poles, called biorientation. Sister chromatid cohesion resists microtubule forces, generating tension which provides the signal that biorientation has occurred. How tension silences the surveillance pathways that prevent cell cycle progression and correct erroneous kinetochore-microtubule attachments remains unclear. Here we show that SUMOylation dampens error correction to allow stable sister kinetochore biorientation and timely anaphase onset. The Siz1/Siz2 SUMO ligases modify the pericentromere-localized shugoshin (Sgo1) protein prior to its tension-dependent release from chromatin. Sgo1 SUMOylation reduces its binding to Protein Phosphatase 2A (PP2A) and weakening of this interaction is important for stable biorientation. Unstable biorientation in SUMO-deficient cells is associated with persistence of the chromosome passenger complex (CPC) at centromeres and SUMOylation of CPC subunit Bir1 also contributes to timely anaphase onset. We propose that SUMOylation acts in a combinatorial manner to facilitate dismantling of the error correction machinery within pericentromeres and thereby sharpen the metaphase-anaphase transition.
Original languageEnglish
Article numbere202005130
Number of pages25
JournalJournal of Cell Biology
Issue number7
Publication statusPublished - 30 Apr 2021


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