Suppression of the Escherichia coli dnaA46 mutation by a mutation in trxA, the gene for thioredoxin

T R Hupp, J M Kaguni

Research output: Contribution to journalArticlepeer-review

Abstract

The dasC mutation, an extragenic suppressor of dnaA46, was mapped by P1 transduction near the rep, trxA, rho region of the Escherichia coli chromosome. The dasC mutation could not be separated from trxA by P1 transduction indicating that dasC and trxA are allelic. Multicopy plasmids containing an intact trxA gene were able to reverse the suppressive effect of the dasC mutation on the dnaA46 mutation. Introduction of a frameshift mutation into the cloned trxA coding region abolished the ability of these recombinant plasmids to reverse the suppressive effect. These results indicate that dasC is allelic with trxA, the gene encoding thioredoxin.
Original languageEnglish
Pages (from-to)471-8
Number of pages8
JournalMolecular and General Genetics MGG
Volume213
Issue number2-3
Publication statusPublished - Aug 1988

Keywords

  • Bacterial Proteins
  • DNA, Bacterial
  • Escherichia coli
  • Genes, Bacterial
  • Mutation
  • Plasmids
  • Recombination, Genetic
  • Restriction Mapping
  • Suppression, Genetic
  • Thioredoxins
  • Transduction, Genetic

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