Surface Enhanced Raman Spectroscopy (SERS) for the detection of intracellular constituents using gold nanoshells - art. no. 609905

Mustafa H. Chowdhury, Colin J. Campbell, Eirini Theofanidou, Seung Joon Lee, Angela Baldwin, Garwin Sing, Alvin T. Yeh, Jason Crain, Peter Ghazal, Gerard L. Cote

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This study reports on current work involving the use of Surface Enhanced Raman Spectroscopy (SERS) for the intracellular detection of cell constituents in mouse fibroblast cells using gold nanoshells. Gold nanoshells were acquired from Nanospectra Biosciences that are based on a silica dielectric core and an outer gold shell layer. They have the unique property of a tunable surface plasmon resonance wavelength from the visible through the near infrared which allows control of the electromagnetic field strength on its surface. Hence gold nanoshells can serve as SERS substrates with plasmonic properties that are not aggregation dependent and thus can be expected to overcome the reproducibility problem that is generally associated with aggregation based colloidal metal nanoparticles. These results represent the first steps in the development of a nanoshell-based SERS probe to detect cell organelles and/or intracellular biochemicals with the goal of ultimately improving the ability to monitor intracellular biological processes in real time.

Original languageEnglish
Title of host publicationPlasmonics in Biology and Medicine III
EditorsV Tuan, JR Lakowicz, Z Gryczynski
Place of PublicationBELLINGHAM
PublisherSPIE
Pages9905-9905
Number of pages8
ISBN (Print)0-8194-6141-5
DOIs
Publication statusPublished - 2006

Keywords / Materials (for Non-textual outputs)

  • Surface Enhanced Raman Spectroscopy (SERS)
  • Raman Spectroscopy
  • gold nanoshells
  • colloidal gold
  • nanoparticles
  • cell
  • intracellular detection
  • LIVING CELLS
  • SCATTERING
  • NANOPARTICLES
  • MICROSCOPY

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