SV40-mediated tumor selection and chromosome transfer to enrich for cystic fibrosis region

D J Porteous, J R Dorin, M M Wilkinson, J M Fletcher, E Emslie, V van Heyningen

Research output: Contribution to journalArticlepeer-review

Abstract

The somatic cell hybrid C121, with chromosome 7 as its sole human component, arose when mouse macrophages SV40 genomes are integrated at 7q31-7q35. We show that hybrids with a reduced chromosome 7 component, but which retain markers linked to the cystic fibrosis locus, can be generated by direct in vivo tumor selection or following chromosome-mediated gene transfer and SV40-mediated cellular transformation. Our methods for chromosome fragmentation and fine-structure mapping can now be applied to the substantial number of SV40-transformed human cell lines, with independent chromosomal integration sites, already available. Our results also suggest that expression of human epidermal growth factor receptor augments the tumorigenic potential of the SV40-transformed C121 hybrid.
Original languageEnglish
Pages (from-to)29-38
Number of pages10
JournalSomatic Cell and Molecular Genetics
Volume16
Issue number1
Publication statusPublished - 1990

Keywords

  • Animals
  • Antigens
  • Cell Line, Transformed
  • Cell Transformation, Neoplastic
  • Cell Transformation, Viral
  • Chromosome Mapping
  • Chromosomes, Human, Pair 7
  • Cystic Fibrosis
  • DNA Probes
  • Genetic Techniques
  • Humans
  • Hybrid Cells
  • Mice
  • Proto-Oncogenes
  • Receptor, Epidermal Growth Factor
  • Repetitive Sequences, Nucleic Acid
  • Simian virus 40
  • Transfection

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