Synergistic effect of co-stimulation of membrane and endosomal TLRs on chicken innate immune responses

Sungwon Kim; Peter Kaiser; Dominika Borowska; Lonneke Vervelde

doi:10.1016/j.vetimm.2018.03.005

Synergistic effect of co-stimulation of membrane and endosomal TLRs on chicken innate immune responses

Sungwon Kim, Peter Kaiser, Dominika Borowska, Lonneke Vervelde

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract

Toll-like receptor (TLR) ligands (TLR-Ls) are critical activators of immunity and are successfully being developed as vaccine adjuvants in both mammals and birds. In this study, we investigated the synergistic effect of co-stimulation of membrane and endosomal TLRs on the innate immune responses using chicken bone marrow-derived macrophages (BMMs), and studied the effect of age on the induction of innate responses. BMMs from 1 and 4-week-old birds were stimulated with Pam3Cys-SK4 (PCSK; TLR2), synthetic monophosphoryl lipid A
(MPLA), Di[3-deoxy-D-manno-octulosonyl]-lipid A ammonium salt (KLA; TLR4),
Gardiquimod, Resiquimod (R848; TLR7), CpG class B and C (TLR21). Nitric oxide (NO) production and mRNA levels of IL-1, IL-10 and IL-12p40 showed macrophages from 4-week-old birds showed more sensitive responses compared to 1-week-old birds. The most potent TLR-Ls, PCSK, MPLA and CpG B were used to study the effect of co-stimulation on macrophages. Co-stimulation with TLR21 and TLR4 synergistically up-regulated inflammatory-related genes, as well as NO production. However, incubation of splenocytes with PCSK, MPLA and CpG B did not induce cell proliferation. Moreover, treatment with CpG
B led to significant cell death.

Original language	English
Pages (from-to)	15-21
Journal	Veterinary Immunology and Immunopathology
Volume	199
Early online date	19 Mar 2018
DOIs	https://doi.org/10.1016/j.vetimm.2018.03.005
Publication status	Published - May 2018

Keywords

Chicken
Toll-like receptor
macrophage
cytokine
adjuvant

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10.1016/j.vetimm.2018.03.005

Synergistic effect of co-stimulation of membrane andAccepted author manuscript, 1.04 MBLicence: CC BY-NC-ND

ISP 2 2017/22 Control of Infectious Diseases
Gally, D.
BBSRC
1/04/17 → 31/03/22
Project: Research
Innate immunity and endemic diseases in livestock species
Collie, D., Beard, P., Bishop, S., Bronsvoort, M., Burt, D., Fitzgerald, R., Freeman, T., Gally, D., Gill, A., Glass, E., Hocking, P., Hope, J., Hume, D., Kaiser, P., Mabbott, N., McLachlan, G., Morrison, L., Stevens, J., Stevens, M. & Watson, M.
BBSRC
1/04/12 → 31/03/17
Project: Research

Cite this

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title = "Synergistic effect of co-stimulation of membrane and endosomal TLRs on chicken innate immune responses",

abstract = "Toll-like receptor (TLR) ligands (TLR-Ls) are critical activators of immunity and are successfully being developed as vaccine adjuvants in both mammals and birds. In this study, we investigated the synergistic effect of co-stimulation of membrane and endosomal TLRs on the innate immune responses using chicken bone marrow-derived macrophages (BMMs), and studied the effect of age on the induction of innate responses. BMMs from 1 and 4-week-old birds were stimulated with Pam3Cys-SK4 (PCSK; TLR2), synthetic monophosphoryl lipid A(MPLA), Di[3-deoxy-D-manno-octulosonyl]-lipid A ammonium salt (KLA; TLR4),Gardiquimod, Resiquimod (R848; TLR7), CpG class B and C (TLR21). Nitric oxide (NO) production and mRNA levels of IL-1, IL-10 and IL-12p40 showed macrophages from 4-week-old birds showed more sensitive responses compared to 1-week-old birds. The most potent TLR-Ls, PCSK, MPLA and CpG B were used to study the effect of co-stimulation on macrophages. Co-stimulation with TLR21 and TLR4 synergistically up-regulated inflammatory-related genes, as well as NO production. However, incubation of splenocytes with PCSK, MPLA and CpG B did not induce cell proliferation. Moreover, treatment with CpGB led to significant cell death.",

keywords = "Chicken, Toll-like receptor, macrophage, cytokine, adjuvant",

author = "Sungwon Kim and Peter Kaiser and Dominika Borowska and Lonneke Vervelde",

year = "2018",

month = may,

doi = "10.1016/j.vetimm.2018.03.005",

language = "English",

volume = "199",

pages = "15--21",

journal = "Veterinary Immunology and Immunopathology",

issn = "0165-2427",

publisher = "Elsevier",

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TY - JOUR

T1 - Synergistic effect of co-stimulation of membrane and endosomal TLRs on chicken innate immune responses

AU - Kim, Sungwon

AU - Kaiser, Peter

AU - Borowska, Dominika

AU - Vervelde, Lonneke

PY - 2018/5

Y1 - 2018/5

N2 - Toll-like receptor (TLR) ligands (TLR-Ls) are critical activators of immunity and are successfully being developed as vaccine adjuvants in both mammals and birds. In this study, we investigated the synergistic effect of co-stimulation of membrane and endosomal TLRs on the innate immune responses using chicken bone marrow-derived macrophages (BMMs), and studied the effect of age on the induction of innate responses. BMMs from 1 and 4-week-old birds were stimulated with Pam3Cys-SK4 (PCSK; TLR2), synthetic monophosphoryl lipid A(MPLA), Di[3-deoxy-D-manno-octulosonyl]-lipid A ammonium salt (KLA; TLR4),Gardiquimod, Resiquimod (R848; TLR7), CpG class B and C (TLR21). Nitric oxide (NO) production and mRNA levels of IL-1, IL-10 and IL-12p40 showed macrophages from 4-week-old birds showed more sensitive responses compared to 1-week-old birds. The most potent TLR-Ls, PCSK, MPLA and CpG B were used to study the effect of co-stimulation on macrophages. Co-stimulation with TLR21 and TLR4 synergistically up-regulated inflammatory-related genes, as well as NO production. However, incubation of splenocytes with PCSK, MPLA and CpG B did not induce cell proliferation. Moreover, treatment with CpGB led to significant cell death.

AB - Toll-like receptor (TLR) ligands (TLR-Ls) are critical activators of immunity and are successfully being developed as vaccine adjuvants in both mammals and birds. In this study, we investigated the synergistic effect of co-stimulation of membrane and endosomal TLRs on the innate immune responses using chicken bone marrow-derived macrophages (BMMs), and studied the effect of age on the induction of innate responses. BMMs from 1 and 4-week-old birds were stimulated with Pam3Cys-SK4 (PCSK; TLR2), synthetic monophosphoryl lipid A(MPLA), Di[3-deoxy-D-manno-octulosonyl]-lipid A ammonium salt (KLA; TLR4),Gardiquimod, Resiquimod (R848; TLR7), CpG class B and C (TLR21). Nitric oxide (NO) production and mRNA levels of IL-1, IL-10 and IL-12p40 showed macrophages from 4-week-old birds showed more sensitive responses compared to 1-week-old birds. The most potent TLR-Ls, PCSK, MPLA and CpG B were used to study the effect of co-stimulation on macrophages. Co-stimulation with TLR21 and TLR4 synergistically up-regulated inflammatory-related genes, as well as NO production. However, incubation of splenocytes with PCSK, MPLA and CpG B did not induce cell proliferation. Moreover, treatment with CpGB led to significant cell death.

KW - Chicken

KW - Toll-like receptor

KW - macrophage

KW - cytokine

KW - adjuvant

U2 - 10.1016/j.vetimm.2018.03.005

DO - 10.1016/j.vetimm.2018.03.005

M3 - Article

VL - 199

SP - 15

EP - 21

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

SN - 0165-2427

ER -

Synergistic effect of co-stimulation of membrane and endosomal TLRs on chicken innate immune responses

Abstract

Keywords

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Projects

ISP 2 2017/22 Control of Infectious Diseases

Innate immunity and endemic diseases in livestock species

Cite this