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Abstract
Topological entanglements severely interfere with important biological processes. For this reason, genomes must be kept unknotted and unlinked during most of a cell cycle. Type II Topoisomerase (TopoII) enzymes play an important role in this process but the precise mechanisms yielding systematic disentanglement of DNA in vivo are not clear. Here we report computational evidence that Structural Maintenance of Chromosomes (SMC) proteins -- such as cohesins and condensins -- can cooperate with TopoII to establish a synergistic mechanism to resolve topological entanglements. SMC-driven loop extrusion (or diffusion) induces the spatial localisation of essential crossings in turn catalysing the simplification of knots and links by TopoII enzymes even in crowded and confined conditions. The mechanism we uncover is universal in that it does not qualitatively depend on the specific substrate, whether DNA or chromatin, or on SMC processivity; we thus argue that this synergy may be at work across organisms and throughout the cell cycle.
Original language | English |
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Pages (from-to) | 8149-8154 |
Number of pages | 6 |
Journal | Proceedings of the National Academy of Sciences (PNAS) |
Volume | 116 |
Issue number | 17 |
Early online date | 8 Apr 2019 |
DOIs | |
Publication status | Published - 23 Apr 2019 |
Keywords / Materials (for Non-textual outputs)
- cond-mat.soft
- physics.bio-ph
- q-bio.BM
- q-bio.SC
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Dive into the research topics of 'Synergy of Topoisomerase and Structural-Maintenance-of-Chromosomes Proteins Creates a Universal Pathway to Simplify Genome Topology'. Together they form a unique fingerprint.Projects
- 1 Finished
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THREEDCELLPHYSICS: The physics of three dimensional chromosome and protein organisation within the cell
1/07/15 → 30/06/20
Project: Research