Synthesis and application of a new cleavable linker for "click"-based affinity chromatography

Felicetta Landi, Conny M Johansson, Dominic J Campopiano, Alison N Hulme

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

A new chemically-cleavable linker has been synthesised for the affinity-independent elution of biomolecules by classical affinity chromatography. This azo-based linker is shown to couple efficiently with "click" derivatised ligands such as biotin propargyl amide through a copper(I)-catalysed Huisgen 1,3-dipolar cycloaddition reaction. Binding to Affi-Gel matrices displaying ligands coupled to the new linker is both efficient and selective. The captured material may be readily released from the resin upon treatment with sodium dithionite. These mild elution conditions have allowed for the efficient isolation of the affinity partner from complex protein mixtures such as those found in fetal bovine serum.
Original languageEnglish
Pages (from-to)56-59
Number of pages4
JournalOrganic & Biomolecular chemistry
Issue number1
Publication statusPublished - 2010

Keywords / Materials (for Non-textual outputs)

  • affinity chromatography
  • cleavable linker
  • dithionite


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