Synthesis and cellular uptake of cell delivering PNA-peptide conjugates

J J  Diaz-Mochon; L  Bialy; J  Watson; R M  Sanchez-Martin; M  Bradley

doi:10.1039/b503777h

Synthesis and cellular uptake of cell delivering PNA-peptide conjugates

J J Diaz-Mochon, L Bialy, J Watson, R M Sanchez-Martin, M Bradley

Research output: Contribution to journal › Article › peer-review

Abstract

The synthesis and cellular uptake of fluorescently labelled PNA-peptide conjugates is described; Dde/Mmt protected PNA monomers, fully orthogonal to Fmoc chemistry, were used to develop a flexible strategy to give Peptide Nucleic Acids conjugated to tri- and hepta-arginine and the short basic Tat(48-57) peptide as examples of cellular penetrating peptides, thereby allowing efficient cellular delivery of PNA into cells.

Original language	English
Pages (from-to)	3316-3318
Number of pages	3
Journal	Chemical Communications
Issue number	26
DOIs	https://doi.org/10.1039/b503777h
Publication status	Published - 2005

Keywords / Materials (for Non-textual outputs)

MEMBRANE-PERMEABLE PEPTIDES
DDE
DNA

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10.1039/b503777h

Interfacing DNA arrays and combinatorial libraries for high-content enzymatic screening
Bradley, M. (Principal Investigator)
Biotechnology and Biological Sciences Research Council
1/03/05 → 31/05/05
Project: Research

Cite this

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title = "Synthesis and cellular uptake of cell delivering PNA-peptide conjugates",

abstract = "The synthesis and cellular uptake of fluorescently labelled PNA-peptide conjugates is described; Dde/Mmt protected PNA monomers, fully orthogonal to Fmoc chemistry, were used to develop a flexible strategy to give Peptide Nucleic Acids conjugated to tri- and hepta-arginine and the short basic Tat(48-57) peptide as examples of cellular penetrating peptides, thereby allowing efficient cellular delivery of PNA into cells.",

keywords = "MEMBRANE-PERMEABLE PEPTIDES, DDE, DNA",

author = "Diaz-Mochon, \{J J\} and L Bialy and J Watson and Sanchez-Martin, \{R M\} and M Bradley",

year = "2005",

doi = "10.1039/b503777h",

language = "English",

pages = "3316--3318",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

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T1 - Synthesis and cellular uptake of cell delivering PNA-peptide conjugates

AU - Diaz-Mochon, J J

AU - Bialy, L

AU - Watson, J

AU - Sanchez-Martin, R M

AU - Bradley, M

PY - 2005

Y1 - 2005

N2 - The synthesis and cellular uptake of fluorescently labelled PNA-peptide conjugates is described; Dde/Mmt protected PNA monomers, fully orthogonal to Fmoc chemistry, were used to develop a flexible strategy to give Peptide Nucleic Acids conjugated to tri- and hepta-arginine and the short basic Tat(48-57) peptide as examples of cellular penetrating peptides, thereby allowing efficient cellular delivery of PNA into cells.

AB - The synthesis and cellular uptake of fluorescently labelled PNA-peptide conjugates is described; Dde/Mmt protected PNA monomers, fully orthogonal to Fmoc chemistry, were used to develop a flexible strategy to give Peptide Nucleic Acids conjugated to tri- and hepta-arginine and the short basic Tat(48-57) peptide as examples of cellular penetrating peptides, thereby allowing efficient cellular delivery of PNA into cells.

KW - MEMBRANE-PERMEABLE PEPTIDES

KW - DDE

KW - DNA

U2 - 10.1039/b503777h

DO - 10.1039/b503777h

M3 - Article

SN - 1359-7345

SP - 3316

EP - 3318

JO - Chemical Communications

JF - Chemical Communications

IS - 26

ER -

Synthesis and cellular uptake of cell delivering PNA-peptide conjugates

Abstract

Keywords / Materials (for Non-textual outputs)

Access to Document

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Projects

Interfacing DNA arrays and combinatorial libraries for high-content enzymatic screening

Cite this