Synthesis and Characterization of Nano Ceria for Biological Applications

Hsueh-Shih Chen*, Julie Mazzolini, Jon Ayres, Josh Rappoport, Jamie Lead, Jon Preece

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract / Description of output

We have synthesized ceria nanoparticles by reacting cerium salts and sodium hydroxide without any additive in order to avoid possible cytotoxicity. The synthesized ceria nanoparticles were with cubic structure according to X-ray diffraction and have diameters between 6 and 10 nm analyzed by transmission electron microscopy. Aggregation behaviors of ceria nanoparticles in DI water and cell culture media have been studied by dynamic light scattering. In de-ionized water, ceria nanoparticles formed a stable colloidal solution with hydrodynamic size similar to 70 nm and zeta potential similar to 47 mV. Dispersion of the nanoparticles in serum-free media was found to result in larger aggregates, while the nanoparticles in serum-containing media form a stable solution, which suggests that proteins in serum and ceria nanoparticles have some interactions. In cellular uptake test, entry of ceria nanoparticles into HeLa cells incubated in serum-containing media at 37 degrees C for 60 min was observed and without cytotoxicity.

Original languageEnglish
Title of host publicationNANO-BIO SENSING, IMAGING, AND SPECTROSCOPY
EditorsSW Kang, SH Park, LP Lee, KB Song, YH Choi
Place of PublicationBELLINGHAM
PublisherSPIE-INT SOC OPTICAL ENGINEERING
Number of pages7
DOIs
Publication statusPublished - 2013
EventConference on Nano-Bio Sensing, Imaging, and Spectroscopy (NBSIS) - Jeju, United Kingdom
Duration: 20 Feb 201323 Feb 2013

Publication series

NameProceedings of SPIE
PublisherSPIE-INT SOC OPTICAL ENGINEERING
Volume8879
ISSN (Print)0277-786X

Conference

ConferenceConference on Nano-Bio Sensing, Imaging, and Spectroscopy (NBSIS)
Country/TerritoryUnited Kingdom
Period20/02/1323/02/13

Keywords / Materials (for Non-textual outputs)

  • Ceria
  • CeO2
  • Hydrothermal
  • Nanoparticles
  • Nanotoxicity
  • Nanorods
  • Nanowires
  • Toxicity
  • QUANTUM DOTS
  • NANOPARTICLES
  • GROWTH

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