Synthesis of DnaK protein during the division cycle of Escherichia coli

T R Hupp, J D Keasling, S Cooper, J M Kaguni

Research output: Contribution to journalArticlepeer-review

Abstract

DnaK protein is involved in the initiation of DNA synthesis from the Escherichia coli chromosome as well as from the replication origins of phage lambda and P1. The synthesis of dnaK mRNA and protein has been reported to vary during the cell cycle of Caulobacter crescentus (Gomes et al., 1990). We have measured the expression of DnaK protein during the E. coli division cycle using the membrane-elution method. Cells labelled with a radioactive amino acid at different times during the division cycle were analysed for radiolabelled DnaK protein by quantitative immunoprecipitation, gel electrophoresis and autoradiography. In contrast to reports of cell-cycle-specific synthesis of DnaK protein in C. crescentus, we find the synthesis of DnaK protein to be invariant during the E. coli division cycle. Its synthesis occurs exponentially, as does the synthesis of total cell protein.
Original languageEnglish
Pages (from-to)99-109
Number of pages11
JournalResearch in microbiology
Volume145
Issue number2
Publication statusPublished - Feb 1994

Keywords

  • Autoradiography
  • Cell Division
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli
  • Escherichia coli Proteins
  • HSP70 Heat-Shock Proteins
  • Heat-Shock Proteins
  • Precipitin Tests

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