Abstract / Description of output
The chromophore 3-Z phycocyanobilin (PCB, (2R,3Z)-8,12-bis(2-carboxyethyl)-18-ethyl-3-ethylidene-2,7,13,17-tetramethyl-2,3-dihydrobilin-1,19(21H,24H)-dione) mediates red and far-red light perception in natural and synthetic biological systems. Here we describe a PCB synthesis strategy in mammalian cells. We optimize the production by co-localizing the biocatalysts to the substrate source, by coordinating the availability of the biocatalysts and by reducing the degradation of the reaction product. We show that the resulting PCB levels of 2 μM are sufficient to sustain the functionality of red light-responsive optogenetic tools suitable for the light-inducible control of gene expression in mammalian cells.
Original language | English |
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Pages (from-to) | 8970-2 |
Number of pages | 3 |
Journal | Chemical Communications |
Volume | 49 |
Issue number | 79 |
DOIs | |
Publication status | Published - 11 Oct 2013 |
Keywords / Materials (for Non-textual outputs)
- animals
- bacterial proteins
- biocatalysis
- CHO cells
- cricetinae
- cricetulus
- cyanobacteria
- heme oxygenase-1
- light
- oxidoreductases Acting on CH-CH Group Donors
- phycobilins
- phycocyanin
- plasmids
- RNA interference
- RNA
- small interfering