Synthetic biology approaches for dynamic CHO cell engineering

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Fed-batch culture of Chinese hamster ovary (CHO) cells remains the most commonly used method for producing biopharmaceuticals. Static CHO cell-line engineering approaches have incrementally improved productivity, growth and product quality through permanent knockout of genes with a negative impact on production, or constitutive overexpression of genes with a positive impact. However, during fed-batch culture, conditions (such as nutrient availability) are continually changing. Therefore, traits that are most beneficial during early-phase culture (such as high growth rate) may be less desirable in late phase. Unlike with static approaches, dynamic cell line engineering strategies can optimise such traits by implementing synthetic sense-and-respond programmes. Here, we review emerging synthetic biology tools that can be used to build dynamic, self-regulating CHO cells, capable of detecting intra-/extracellular cues and generating user-defined responses tailored to the stage-specific needs of the production process.
Original languageEnglish
Article number102806
JournalCurrent opinion in biotechnology
Volume78
Early online date1 Oct 2022
DOIs
Publication statusPublished - 1 Dec 2022

Keywords / Materials (for Non-textual outputs)

  • Chinese hamster ovary cell (CHO)
  • cell line engineering
  • synthetic biology
  • engineering biology
  • CHO production
  • dynamic engineering
  • self-regulating
  • biotherapeutics
  • quorum sensing
  • lactate metabolism
  • gene circuits
  • synthetic receptors
  • synthetic promoters

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