Synthetic lethality with fibrillarin identifies NOP77p, a nucleolar protein required for pre-rRNA processing and modification

Thierry Bergès, Elisabeth Petfalski, David Tollervey, Eduard C. Hurt*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The nucleolar protein fibrillarin (encoded by the NOP1 gene in yeast), is required for many post-transcriptional steps in yeast ribosome synthesis. A screen for mutations showing synthetic lethality with a temperature sensitive nopl-5 allele led to the identification of the NOP77 gene. NOP77 is essential for viability and encodes a nucleolar protein with a predicted molecular weight of 77 kDa. Depletion of NOP77p impairs both the processing and methylation of the pre-rRNA. The processing defect is greatest for the pathway leading to 25S rRNA synthesis, and is distinctly different from that observed for mutations in other nucleolar components. NOP77p contains three canonical RNA recognition motifs (RRMs), suggesting that it is an RNA binding protein. The NOP77 allele which complements the synthetic lethal nop1 strains has an alanine at position 308, predicted to lie in helix α1 of RRM3, whereas the non-complementing nop77-1 allele contains a proline at the corresponding position. We propose that NOP77p mediates specific interactions between NOP1p and the pre-rRNA.

Original languageEnglish
Pages (from-to)3136-3148
Number of pages13
JournalEMBO Journal
Volume13
Issue number13
Publication statusPublished - 25 Jul 1994

Keywords / Materials (for Non-textual outputs)

  • Fibrillarin
  • NOP1
  • Nucleolus
  • RNA binding
  • Yeast

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