Targeted genome editing across species using ZFNs and TALENs

Andrew Wood, Te-Wen Lo, Bryan Zeitler, Catherine S Pickle, Edward J Ralston, Andrew H Lee, Rainier Amora, Jeffrey C Miller, Elo Leung, Xiangdong Meng, Lei Zhang, Edward J Rebar, Philip D Gregory, Fyodor D Urnov, Barbara J Meyer

Research output: Contribution to journalArticlepeer-review

Abstract

Evolutionary studies necessary to dissect diverse biological processes have been limited by the lack of reverse genetic approaches in most organisms with sequenced genomes. We established a broadly applicable strategy using zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) for targeted disruption of endogenous genes and cis-acting regulatory elements in diverged nematode species.
Original languageEnglish
Pages (from-to)307
JournalScience
Volume333
Issue number6040
DOIs
Publication statusPublished - 15 Jul 2011

Keywords

  • Animals
  • Regulatory Elements, Transcriptional
  • Transgenes
  • Genome, Helminth
  • INDEL Mutation
  • Mutagenesis
  • Caenorhabditis
  • Transcription Factors
  • Genetic Techniques
  • Caenorhabditis elegans
  • Zinc Fingers
  • Deoxyribonucleases, Type II Site-Specific
  • Genes, Helminth
  • Gene Targeting

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