Targeted genome editing across species using ZFNs and TALENs

Andrew Wood; Te-Wen Lo; Bryan Zeitler; Catherine S Pickle; Edward J Ralston; Andrew H Lee; Rainier Amora; Jeffrey C Miller; Elo Leung; Xiangdong Meng; Lei Zhang; Edward J Rebar; Philip D Gregory; Fyodor D Urnov; Barbara J Meyer

doi:10.1126/science.1207773

Targeted genome editing across species using ZFNs and TALENs

Andrew Wood, Te-Wen Lo, Bryan Zeitler, Catherine S Pickle, Edward J Ralston, Andrew H Lee, Rainier Amora, Jeffrey C Miller, Elo Leung, Xiangdong Meng, Lei Zhang, Edward J Rebar, Philip D Gregory, Fyodor D Urnov, Barbara J Meyer

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Evolutionary studies necessary to dissect diverse biological processes have been limited by the lack of reverse genetic approaches in most organisms with sequenced genomes. We established a broadly applicable strategy using zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) for targeted disruption of endogenous genes and cis-acting regulatory elements in diverged nematode species.

Original language	English
Pages (from-to)	307
Journal	Science
Volume	333
Issue number	6040
DOIs	https://doi.org/10.1126/science.1207773
Publication status	Published - 15 Jul 2011

Keywords / Materials (for Non-textual outputs)

Animals
Regulatory Elements, Transcriptional
Transgenes
Genome, Helminth
INDEL Mutation
Mutagenesis
Caenorhabditis
Transcription Factors
Genetic Techniques
Caenorhabditis elegans
Zinc Fingers
Deoxyribonucleases, Type II Site-Specific
Genes, Helminth
Gene Targeting

Access to Document

10.1126/science.1207773

Targeted genome editing across species using ZFNs and TALENs
NIH Public access author's manuscript
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http://www.sciencemag.org/content/333/6040/307

Cite this

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title = "Targeted genome editing across species using ZFNs and TALENs",

abstract = "Evolutionary studies necessary to dissect diverse biological processes have been limited by the lack of reverse genetic approaches in most organisms with sequenced genomes. We established a broadly applicable strategy using zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) for targeted disruption of endogenous genes and cis-acting regulatory elements in diverged nematode species.",

keywords = "Animals, Regulatory Elements, Transcriptional, Transgenes, Genome, Helminth, INDEL Mutation, Mutagenesis, Caenorhabditis, Transcription Factors, Genetic Techniques, Caenorhabditis elegans, Zinc Fingers, Deoxyribonucleases, Type II Site-Specific, Genes, Helminth, Gene Targeting",

author = "Andrew Wood and Te-Wen Lo and Bryan Zeitler and Pickle, {Catherine S} and Ralston, {Edward J} and Lee, {Andrew H} and Rainier Amora and Miller, {Jeffrey C} and Elo Leung and Xiangdong Meng and Lei Zhang and Rebar, {Edward J} and Gregory, {Philip D} and Urnov, {Fyodor D} and Meyer, {Barbara J}",

year = "2011",

month = jul,

day = "15",

doi = "10.1126/science.1207773",

language = "English",

volume = "333",

pages = "307",

journal = "Science",

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T1 - Targeted genome editing across species using ZFNs and TALENs

AU - Wood, Andrew

AU - Lo, Te-Wen

AU - Zeitler, Bryan

AU - Pickle, Catherine S

AU - Ralston, Edward J

AU - Lee, Andrew H

AU - Amora, Rainier

AU - Miller, Jeffrey C

AU - Leung, Elo

AU - Meng, Xiangdong

AU - Zhang, Lei

AU - Rebar, Edward J

AU - Gregory, Philip D

AU - Urnov, Fyodor D

AU - Meyer, Barbara J

PY - 2011/7/15

Y1 - 2011/7/15

N2 - Evolutionary studies necessary to dissect diverse biological processes have been limited by the lack of reverse genetic approaches in most organisms with sequenced genomes. We established a broadly applicable strategy using zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) for targeted disruption of endogenous genes and cis-acting regulatory elements in diverged nematode species.

AB - Evolutionary studies necessary to dissect diverse biological processes have been limited by the lack of reverse genetic approaches in most organisms with sequenced genomes. We established a broadly applicable strategy using zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) for targeted disruption of endogenous genes and cis-acting regulatory elements in diverged nematode species.

KW - Animals

KW - Regulatory Elements, Transcriptional

KW - Transgenes

KW - Genome, Helminth

KW - INDEL Mutation

KW - Mutagenesis

KW - Caenorhabditis

KW - Transcription Factors

KW - Genetic Techniques

KW - Caenorhabditis elegans

KW - Zinc Fingers

KW - Deoxyribonucleases, Type II Site-Specific

KW - Genes, Helminth

KW - Gene Targeting

U2 - 10.1126/science.1207773

DO - 10.1126/science.1207773

M3 - Article

C2 - 21700836

VL - 333

SP - 307

JO - Science

JF - Science

IS - 6040

ER -

Targeted genome editing across species using ZFNs and TALENs

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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