Targeted sequencing of the Paget's disease associated 14q32 locus identifies several missense coding variants in RIN3 that predispose to Paget's disease of bone

Maheva Vallet, Dinesh C Soares, Sachin Wani, Antonia Sophocleous, Jon Warner, Donald M Salter, Stuart H Ralston, Omar M E Albagha

Research output: Contribution to journalArticlepeer-review

Abstract

Paget's disease of bone (PDB) is a common disorder with a strong genetic component characterised by increased but disorganised bone remodelling. Previous genome-wide association studies identified a locus on chromosome 14q32 tagged by rs10498635 which was significantly associated with susceptibility to PDB in several European populations. Here we conducted fine-mapping and targeted sequencing of the candidate locus to identify possible functional variants. Imputation in 741 PDB patients and 2,699 controls confirmed that the association was confined to a 60 kb region in the RIN3 gene and conditional analysis adjusting for rs10498635 identified no new independent signals. Sequencing of the RIN3 gene identified a common missense variant (p.R279C) that was strongly associated with the disease (OR=0.64; P=1.4 x 10(-9)), and was in strong linkage disequilibrium with rs10498635. A further 13 rare missense variants were identified, seven of which were novel and detected only in PDB cases. When combined, these rare variants were over-represented in cases compared to controls (OR=3.72; P=8.9 x 10(-10)). Most rare variants were located in a region that encodes a proline-rich, intrinsically disordered domain of the protein and many were predicted to be pathogenic. RIN3 was expressed in bone tissue and its expression level was ∼10 fold higher in osteoclasts compared to osteoblasts. We conclude that susceptibility to PDB at the 14q32 locus is mediated by a combination of common and rare coding variants in RIN3 and suggest that RIN3 may contribute to PDB susceptibility by affecting osteoclast function.

Original languageEnglish
JournalHuman Molecular Genetics
Early online date20 Feb 2015
DOIs
Publication statusPublished - 1 Jun 2015

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