Projects per year
Abstract
Use of multicellular tumor spheroids (MTS) to investigate therapies has gained impetus because they have potential to mimic factors including zonation, hypoxia and drug-resistance. However, analysis remains difficult and often destroys 3D integrity. Here we report an optical technique using targeted nanosensors that allows in situ 3D mapping of redox potential gradients whilst retaining MTS morphology and function. The magnitude of the redox potential gradient can be quantified as a free energy difference (ΔG) and used as a measurement of MTS viability. We found that by delivering different doses of radiotherapy to MTS we could correlate loss of ΔG with increasing therapeutic dose. In addition, we found that resistance to drug therapy was indicated by an increase in ΔG. This robust and reproducible technique allows interrogation of an in vitro tumor-model's bioenergetic response to therapy, indicating its potential as a tool for therapy development.
Original language | English |
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Pages (from-to) | 16710-16718 |
Number of pages | 8 |
Journal | Nanoscale |
Volume | 8 |
Issue number | 37 |
Early online date | 6 Sept 2016 |
DOIs | |
Publication status | Published - 6 Sept 2016 |
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Dive into the research topics of 'Targeted SERS nanosensors measure physicochemical gradients and free energy changes in live 3D tumor spheroids'. Together they form a unique fingerprint.Projects
- 1 Finished
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Mapping the local state redox potentials with subcellular resolution
1/11/12 → 29/02/16
Project: Research
Profiles
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Colin Campbell
- School of Chemistry - Personal Chair of Medical and Biological Spectroscopy
- EaStCHEM
Person: Academic: Research Active