Technoeconomic comparison of optimised bioreactor-filtration systems for mAb production

Wil Jones, Dimitrios Gerogiorgis*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Primary post-cultivation biomass recovery (cell culture removal from API-rich solutions) is essential in biopharmaceutical manufacturing. Centrifugation and depth filtration are dominant industrial primary recovery technologies, but mechanistic dynamic models suitable for performance evaluation are scarce. This paper uses established literature models to present and analyse optimal operation strategies for integrated process designs of fed-batch or perfusion CHO bioreactors (for mAb cultivation via CHO cultures), with an explicit rotational disk (dynamic crossflow) filtration model (for primary recovery). A rigorous DAE filter model (Marke et al., 2020) is employed here, to evaluate system performance. Dynamic optimisation of bioreactor-filter systems has been completed for different bioreactor types, filter arrangements and feed manipulations, considering the same annual mAb plant production target. A technoeconomic analysis of optimal designs addresses industrial viability, confirming a clear cost advantage of fed-batch reactors combined with stacked membrane microfilters.
Original languageEnglish
Article number108438
Number of pages46
JournalComputers and Chemical Engineering
Early online date24 Oct 2023
Publication statusPublished - Nov 2023

Keywords / Materials (for Non-textual outputs)

  • Dynamic simulation
  • Dynamic optimisation
  • technoeconomic analysis
  • Biopharmaceutical manufacturing


Dive into the research topics of 'Technoeconomic comparison of optimised bioreactor-filtration systems for mAb production'. Together they form a unique fingerprint.

Cite this