Tel2 mediates activation and localization of ATM/Tel1 kinase to a double-strand break

Carol M Anderson, Dmitry Korkin, Dana L Smith, Svetlana Makovets, Jeffrey J Seidel, Andrej Sali, Elizabeth H Blackburn

Research output: Contribution to journalArticlepeer-review

Abstract

The kinases ATM and ATR (Tel1 and Mec1 in the yeast Saccharomyces cerevisiae) control the response to DNA damage. We report that S. cerevisiae Tel2 acts at an early step of the TEL1/ATM pathway of DNA damage signaling. We show that Tel1 and Tel2 interact, and that even when Tel1 protein levels are high, this interaction is specifically required for Tel1 localization to a DNA break and its activation of downstream targets. Computational analysis revealed structural homology between Tel2 and Ddc2 (ATRIP in vertebrates), a partner of Mec1, suggesting a common structural principle used by partners of phoshoinositide 3-kinase-like kinases.
Original languageEnglish
Pages (from-to)854-859
Number of pages6
JournalGenes & Development
Volume22
Issue number7
DOIs
Publication statusPublished - 2008

Keywords

  • DNA damage signaling
  • checkpoint
  • Clk-2
  • PI3 kinase-like kinase
  • α-superhelical protein
  • telomere

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