Test of candidate gene--quantitative trait locus association applied to fatness in mice

P D Keightley, K H Morris, A Ishikawa, V M Falconer, F Oliver

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

We test for the contribution of five strong candidate genes for obesity to quantitative variation for fatness in mice. The candidate loci are known through their major mutant phenotypes. We propose a randomization test for overall contribution of candidate genes, based on the empirical distribution of LOD scores from a quantitative trait locus (QTL) genome scan. The test is applied to data on body fat content and male gonadal fatpad weight from a QTL genome scan with an F2 population of C57BL/6J and DBA/2J inbred mice. The test is nonsignificant in this experiment for overall body fat content. QTLs detected at an experiment-wide significance level on chromosome 4, 6, 13 and 15 have effects on mean fatness of up to 19% between the homozygotes, but map to locations where there is no strong candidate gene. The test is significant for gonadal fat pad weight in males, and gives weak support for an association with the diabetes gene.

Original languageEnglish
Pages (from-to)630-7
Number of pages8
JournalHeredity
Volume81 ( Pt 6)
DOIs
Publication statusPublished - Dec 1998

Keywords / Materials (for Non-textual outputs)

  • Adipose Tissue
  • Animals
  • Chromosome Mapping
  • Crosses, Genetic
  • Female
  • Genetic Linkage
  • Homozygote
  • Hybridization, Genetic
  • Likelihood Functions
  • Lod Score
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred DBA
  • Mutation
  • Obesity
  • Quantitative Trait, Heritable

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