TEX15 is an essential executor of MIWI2-directed transposon DNA methylation and silencing

Theresa Schöpp, Ansgar Zoch, Rebecca V. Berrens, Tania Auchynnikava, Yuka Kabayama, Lina Vasiliauskaitė, Juri Rappsilber, Robin C. Allshire, Donal O'Carroll

Research output: Contribution to journalArticlepeer-review

Abstract

The PIWI protein MIWI2 and its associated PIWI-interacting RNAs (piRNAs) instruct DNA methylation of young active transposable elements (TEs) in the male germline. PiRNAs are proposed to recruit MIWI2 to the transcriptionally active TE loci by base pairing to nascent transcripts, however the downstream mechanisms and effector proteins utilized by MIWI2 in directing de novo TE methylation remain incompletely understood. Here we show that MIWI2 associates with TEX15 in foetal gonocytes. TEX15 is predominantly a nuclear protein that is not required for piRNA biogenesis but is essential for piRNA-directed TE de novo methylation and silencing. In summary, TEX15 is an essential executor of mammalian piRNA-directed DNA methylation.
Original languageEnglish
Article number3739
JournalNature Communications
Volume11
Early online date27 Jul 2020
DOIs
Publication statusE-pub ahead of print - 27 Jul 2020

Fingerprint Dive into the research topics of 'TEX15 is an essential executor of MIWI2-directed transposon DNA methylation and silencing'. Together they form a unique fingerprint.

Cite this