TEX15 is an essential executor of MIWI2-directed transposon DNA methylation and silencing

Theresa Schöpp; Ansgar Zoch; Rebecca V. Berrens; Tania Auchynnikava; Yuka Kabayama; Lina Vasiliauskaitė; Juri Rappsilber; Robin C.  Allshire; Donal O'Carroll

doi:10.1038/s41467-020-17372-5

TEX15 is an essential executor of MIWI2-directed transposon DNA methylation and silencing

Theresa Schöpp, Ansgar Zoch, Rebecca V. Berrens, Tania Auchynnikava, Yuka Kabayama, Lina Vasiliauskaitė, Juri Rappsilber, Robin C. Allshire, Donal O'Carroll

Research output: Contribution to journal › Article › peer-review

Abstract

The PIWI protein MIWI2 and its associated PIWI-interacting RNAs (piRNAs) instruct DNA methylation of young active transposable elements (TEs) in the male germline. PiRNAs are proposed to recruit MIWI2 to the transcriptionally active TE loci by base pairing to nascent transcripts, however the downstream mechanisms and effector proteins utilized by MIWI2 in directing de novo TE methylation remain incompletely understood. Here we show that MIWI2 associates with TEX15 in foetal gonocytes. TEX15 is predominantly a nuclear protein that is not required for piRNA biogenesis but is essential for piRNA-directed TE de novo methylation and silencing. In summary, TEX15 is an essential executor of mammalian piRNA-directed DNA methylation.

Original language	English
Article number	3739
Journal	Nature Communications
Volume	11
Early online date	27 Jul 2020
DOIs	https://doi.org/10.1038/s41467-020-17372-5
Publication status	E-pub ahead of print - 27 Jul 2020

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10.1038/s41467-020-17372-5Licence: Creative Commons: Attribution (CC-BY)

O'CarrollEtalNC2020TEX15IsAnEssentialExecutorOfMIWI2DirectedFinal published version, 1.85 MBLicence: Creative Commons: Attribution (CC-BY)

Epigenetic inheritance: establishment and transmission of specialised chromatin domains
Allshire, R.
UK-based charities
1/04/17 → 31/03/23
Project: Research
Wellcome Centre for Cell Biology
Tollervey, D.
UK-based charities
1/12/16 → 1/12/21
Project: Research
Proteomics at the Wellcome Trust Centre for Cell Biology (WTCCB) and School of Biological Sciences (SBS), Edinburgh
Rappsilber, J.
UK-based charities
1/10/15 → 30/09/20
Project: Research

Institute for Regeneration and Repair Flow Cytometry Facility
Shonna Johnston (Manager), Fiona Rossi (Manager), Claire Cryer (Other) & Ailsa Laird (Other)
Institute of Regeneration and Repair
Facility/equipment: Facility

Donal O'Carroll
- School of Biological Sciences - Chair of Stem Cell Biology
- Centre for Regenerative Medicine
Person: Academic: Research Active

Cite this

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title = "TEX15 is an essential executor of MIWI2-directed transposon DNA methylation and silencing",

abstract = "The PIWI protein MIWI2 and its associated PIWI-interacting RNAs (piRNAs) instruct DNA methylation of young active transposable elements (TEs) in the male germline. PiRNAs are proposed to recruit MIWI2 to the transcriptionally active TE loci by base pairing to nascent transcripts, however the downstream mechanisms and effector proteins utilized by MIWI2 in directing de novo TE methylation remain incompletely understood. Here we show that MIWI2 associates with TEX15 in foetal gonocytes. TEX15 is predominantly a nuclear protein that is not required for piRNA biogenesis but is essential for piRNA-directed TE de novo methylation and silencing. In summary, TEX15 is an essential executor of mammalian piRNA-directed DNA methylation.",

author = "Theresa Sch{\"o}pp and Ansgar Zoch and Berrens, {Rebecca V.} and Tania Auchynnikava and Yuka Kabayama and Lina Vasiliauskaitė and Juri Rappsilber and Allshire, {Robin C.} and Donal O'Carroll",

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year = "2020",

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doi = "10.1038/s41467-020-17372-5",

language = "English",

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journal = "Nature Communications",

issn = "2041-1723",

publisher = "Nature Publishing Group",

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TY - JOUR

T1 - TEX15 is an essential executor of MIWI2-directed transposon DNA methylation and silencing

AU - Schöpp, Theresa

AU - Zoch, Ansgar

AU - Berrens, Rebecca V.

AU - Auchynnikava, Tania

AU - Kabayama, Yuka

AU - Vasiliauskaitė, Lina

AU - Rappsilber, Juri

AU - Allshire, Robin C.

AU - O'Carroll, Donal

N1 - Gold OA

PY - 2020/7/27

Y1 - 2020/7/27

N2 - The PIWI protein MIWI2 and its associated PIWI-interacting RNAs (piRNAs) instruct DNA methylation of young active transposable elements (TEs) in the male germline. PiRNAs are proposed to recruit MIWI2 to the transcriptionally active TE loci by base pairing to nascent transcripts, however the downstream mechanisms and effector proteins utilized by MIWI2 in directing de novo TE methylation remain incompletely understood. Here we show that MIWI2 associates with TEX15 in foetal gonocytes. TEX15 is predominantly a nuclear protein that is not required for piRNA biogenesis but is essential for piRNA-directed TE de novo methylation and silencing. In summary, TEX15 is an essential executor of mammalian piRNA-directed DNA methylation.

AB - The PIWI protein MIWI2 and its associated PIWI-interacting RNAs (piRNAs) instruct DNA methylation of young active transposable elements (TEs) in the male germline. PiRNAs are proposed to recruit MIWI2 to the transcriptionally active TE loci by base pairing to nascent transcripts, however the downstream mechanisms and effector proteins utilized by MIWI2 in directing de novo TE methylation remain incompletely understood. Here we show that MIWI2 associates with TEX15 in foetal gonocytes. TEX15 is predominantly a nuclear protein that is not required for piRNA biogenesis but is essential for piRNA-directed TE de novo methylation and silencing. In summary, TEX15 is an essential executor of mammalian piRNA-directed DNA methylation.

U2 - 10.1038/s41467-020-17372-5

DO - 10.1038/s41467-020-17372-5

M3 - Article

SN - 2041-1723

VL - 11

JO - Nature Communications

JF - Nature Communications

M1 - 3739

ER -

TEX15 is an essential executor of MIWI2-directed transposon DNA methylation and silencing

Abstract

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Projects

Epigenetic inheritance: establishment and transmission of specialised chromatin domains

Wellcome Centre for Cell Biology

Proteomics at the Wellcome Trust Centre for Cell Biology (WTCCB) and School of Biological Sciences (SBS), Edinburgh

Equipment

Institute for Regeneration and Repair Flow Cytometry Facility

Profiles

Donal O'Carroll

Cite this