The 3′ processing of antisense RNAs physically links to chromatin-based transcriptional control

Xiaofeng Fang, Zhe Wu, Oleg Raitskin, Kimberly Webb, Philipp Voigt, Tiancong Lu, Martin Howard, Caroline Dean

Research output: Contribution to journalArticlepeer-review

Abstract

Noncoding RNA plays essential roles in transcriptional control and chromatin silencing. At Arabidopsis thaliana FLC, antisense transcription quantitatively influences transcriptional output, but the mechanism by which this occurs is still unclear. Proximal polyadenylation of the antisense transcripts by FCA, an RNA-binding protein that physically interacts with RNA 3′ processing factors, reduces FLC transcription. This process genetically requires FLD, a homolog of the H3K4 demethylase LSD1. However, the mechanism linking RNA processing to FLD function had not been established. Here, we show that FLD tightly associates with LUMINIDEPENDENS (LD) and SET DOMAIN GROUP 26 (SDG26) in vivo, and, together, they prevent accumulation of monomethylated H3K4 (H3K4me1) over the FLC gene body. SDG26 interacts with the RNA 3′ processing factor FY (WDR33),thus linking activities for proximal polyadenylation of the antisense transcripts to FLD/LD/SDG26-associated H3K4 demethylation. We propose this demethylation antagonizes an active transcription module, thus reducing H3K36me3 accumulation and increasing H3K27me3. Consistent with this view, we show that Polycomb Repressive Complex 2(PRC2) silencing is genetically required by FCA to repress FLC. Overall, our work provides insights into RNA-mediated chromatin silencing.
Original languageEnglish
Pages (from-to)15316-15321
Number of pages6
JournalProceedings of the National Academy of Sciences
Volume117
Issue number26
Early online date15 Jun 2020
DOIs
Publication statusPublished - 30 Jun 2020

Keywords

  • non coding RNA
  • chromatin
  • polycomb
  • FLC
  • arabidopsis

Fingerprint Dive into the research topics of 'The 3′ processing of antisense RNAs physically links to chromatin-based transcriptional control'. Together they form a unique fingerprint.

Cite this