TY - JOUR
T1 - The ABCA1 cholesterol transporter associates with one of two distinct dystrophin-based scaffolds in Schwann cells
AU - Albrecht, D. E.
AU - Sherman, D. L.
AU - Brophy, P. J.
AU - Froehner, S. C.
PY - 2008/4
Y1 - 2008/4
N2 - Cytoskeletal scaffolding complexes help organize specialized membrane domains with unique functions on the surface of cells. In this study, we define the scaffolding potential of the Schwarm cell dystrophin glycoprotein complex (DGC) by establishing the presence of four syntrophin isoforms, (alpha 1, beta 1, beta 2, and gamma 2), and one dystrobrevin isoform, (alpha-dystrobrevin-1), in the abaxonal membrane. Furthermore, we demonstrate the existence of two separate DGCs in Schwarm cells that divide the abaxonal membrane into spatially distinct domains, the DRP2/periaxin rich plaques and the Cajal bands that contain Dp116, utrophin, alpha-dystrobrevin-1 and four syntrophin isoforms. Finally, we show that the two different DGCs can scaffold unique accessory molecules in distinct areas of the Schwarm cell membrane. Specifically, the cholesterol transporter ABCA1, associates with the Dp116/syntrophin complex in Cajal bands and is excluded from the DRP2/periaxin rich plaques. (C) 2008 Wiley-Liss, Inc.
AB - Cytoskeletal scaffolding complexes help organize specialized membrane domains with unique functions on the surface of cells. In this study, we define the scaffolding potential of the Schwarm cell dystrophin glycoprotein complex (DGC) by establishing the presence of four syntrophin isoforms, (alpha 1, beta 1, beta 2, and gamma 2), and one dystrobrevin isoform, (alpha-dystrobrevin-1), in the abaxonal membrane. Furthermore, we demonstrate the existence of two separate DGCs in Schwarm cells that divide the abaxonal membrane into spatially distinct domains, the DRP2/periaxin rich plaques and the Cajal bands that contain Dp116, utrophin, alpha-dystrobrevin-1 and four syntrophin isoforms. Finally, we show that the two different DGCs can scaffold unique accessory molecules in distinct areas of the Schwarm cell membrane. Specifically, the cholesterol transporter ABCA1, associates with the Dp116/syntrophin complex in Cajal bands and is excluded from the DRP2/periaxin rich plaques. (C) 2008 Wiley-Liss, Inc.
UR - http://www.scopus.com/inward/record.url?scp=45549096100&partnerID=8YFLogxK
U2 - 10.1002/glia.20636
DO - 10.1002/glia.20636
M3 - Article
SN - 0894-1491
VL - 56
SP - 611
EP - 618
JO - Glia
JF - Glia
IS - 6
ER -