Projects per year
Abstract
Context: Inadequate progesterone production from the corpus luteum is associated with pregnancy loss. Data available in model species suggest important roles of miRNAs in luteal development and maintenance.
Objective: To comprehensively investigate the involvement of miRNAs during the ovarian follicle- luteal transition
Design: The effects of specific miRNAs on survival and steroid production by human luteinized granulosa cells (hLGCs) were tested using specific miRNA inhibitors. Candidate miRNAs were first identified through microarray analyses of follicular and luteal tissues in a bovine model.
Setting: UK academic institution associated with teaching hospital
Patients or other participants: hLGCs were obtained by standard transvaginal follicular fluid aspiration from 35 women undergoing assisted conception
Intervention(s): Inhibition of candidate miRNAs in vitro
Main outcome measure(s): Levels of miRNAs, mRNAs, FOXO1 protein, apoptosis and steroids were measured in tissues and/or cultured cells.
Results: Two specific miRNA clusters, miR-183-96-182 and miR-212-132, were dramatically increased in luteal relative to follicular tissues. miR-96 and miR-132 were the most upregulated miRNAs within each cluster. Database analyses identified FOXO1 as a putative target of both these miRNAs. In cultured hLGCs, inhibition of miR-96 increased apoptosis and FOXO1 protein levels, and decreased progesterone production. These effects were prevented by siRNA-mediated downregulation of FOXO1. In bovine luteal cells, miR-96 inhibition also led to increases in apoptosis and FOXO1 protein levels.
Conclusions: miR-96 targets FOXO1 to regulate luteal development through effects on cell survival and steroid production. The miR-183-96-182 cluster could provide a novel target for the manipulation of luteal function.
Objective: To comprehensively investigate the involvement of miRNAs during the ovarian follicle- luteal transition
Design: The effects of specific miRNAs on survival and steroid production by human luteinized granulosa cells (hLGCs) were tested using specific miRNA inhibitors. Candidate miRNAs were first identified through microarray analyses of follicular and luteal tissues in a bovine model.
Setting: UK academic institution associated with teaching hospital
Patients or other participants: hLGCs were obtained by standard transvaginal follicular fluid aspiration from 35 women undergoing assisted conception
Intervention(s): Inhibition of candidate miRNAs in vitro
Main outcome measure(s): Levels of miRNAs, mRNAs, FOXO1 protein, apoptosis and steroids were measured in tissues and/or cultured cells.
Results: Two specific miRNA clusters, miR-183-96-182 and miR-212-132, were dramatically increased in luteal relative to follicular tissues. miR-96 and miR-132 were the most upregulated miRNAs within each cluster. Database analyses identified FOXO1 as a putative target of both these miRNAs. In cultured hLGCs, inhibition of miR-96 increased apoptosis and FOXO1 protein levels, and decreased progesterone production. These effects were prevented by siRNA-mediated downregulation of FOXO1. In bovine luteal cells, miR-96 inhibition also led to increases in apoptosis and FOXO1 protein levels.
Conclusions: miR-96 targets FOXO1 to regulate luteal development through effects on cell survival and steroid production. The miR-183-96-182 cluster could provide a novel target for the manipulation of luteal function.
| Original language | English |
|---|---|
| Pages (from-to) | 2188-2198 |
| Journal | The Journal of Clinical Endocrinology & Metabolism (JCEM) |
| Volume | 102 |
| Issue number | 7 |
| Early online date | 20 Mar 2017 |
| DOIs | |
| Publication status | Published - 1 Jul 2017 |
Keywords / Materials (for Non-textual outputs)
- miRNA
- miR-96
- ovary
- progesterone
- FOXO1
- luteal cells
- luteinized granulosa cells
Fingerprint
Dive into the research topics of 'The adequate corpus luteum: miR-96 promotes luteal cell survival and progesterone production'. Together they form a unique fingerprint.Projects
- 2 Finished
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Identification of embryonic mirnas and their potential as biomarkers of early pregnancy in cattle
Donadeu, X. (Principal Investigator)
1/11/12 → 31/10/16
Project: Research
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Control of development and reproductive traits
Burdon, T. (Principal Investigator), Argyle, D. (Co-investigator), Ashworth, C. (Co-investigator), Beard, P. (Co-investigator), Brunton, P. (Co-investigator), Burt, D. (Co-investigator), Clinton, M. (Co-investigator), Dunn, I. (Co-investigator), Farquharson, C. (Co-investigator), Headon, D. (Co-investigator), Hocking, P. (Co-investigator), Hohenstein, P. (Co-investigator), Hume, D. (Co-investigator), Jackson, I. (Co-investigator), McColl, B. (Co-investigator), McGrew, M. (Co-investigator), McLachlan, G. (Co-investigator), Sang, H. (Co-investigator), Summers, K. (Co-investigator) & Whitelaw, B. (Co-investigator)
1/04/12 → 31/03/17
Project: Research
Profiles
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Xavier Donadeu
- Royal (Dick) School of Veterinary Studies - Personal Chair of Translational Farm Animal Biology
- Euan MacDonald Centre for Motor Neuron Disease Research
Person: Academic: Research Active