The calvarial injection assay

Robert J van 't Hof

Research output: Contribution to journalArticlepeer-review


This chapter describes the calvarial injection method, whereby the effect of a substance on bone is tested by subcutaneous injection over the calvarium of a mouse. This assay allows testing of the effect of substances on both bone resorption and bone formation in a relatively simple in vivo model. The analysis is carried out by histological means, usually in glycolmethacrylate-embedded tissue, allowing for histochemical analysis and for a variety of different histological staining methods which are also described in detail.
Original languageEnglish
Pages (from-to)537-44
Number of pages8
JournalMethods in Molecular Biology
Publication statusPublished - 2012


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