Abstract / Description of output
This chapter describes the calvarial injection method, whereby the effect of a substance on bone is tested by subcutaneous injection over the calvarium of a mouse. This assay allows testing of the effect of substances on both bone resorption and bone formation in a relatively simple in vivo model. The analysis is carried out by histological means, usually in glycolmethacrylate-embedded tissue, allowing for histochemical analysis and for a variety of different histological staining methods which are also described in detail.
| Original language | English |
|---|---|
| Pages (from-to) | 537-44 |
| Number of pages | 8 |
| Journal | Methods in Molecular Biology |
| Volume | 816 |
| DOIs | |
| Publication status | Published - 2012 |