TY - JOUR
T1 - The calvarial injection assay
AU - van 't Hof, Robert J
PY - 2012
Y1 - 2012
N2 - This chapter describes the calvarial injection method, whereby the effect of a substance on bone is tested by subcutaneous injection over the calvarium of a mouse. This assay allows testing of the effect of substances on both bone resorption and bone formation in a relatively simple in vivo model. The analysis is carried out by histological means, usually in glycolmethacrylate-embedded tissue, allowing for histochemical analysis and for a variety of different histological staining methods which are also described in detail.
AB - This chapter describes the calvarial injection method, whereby the effect of a substance on bone is tested by subcutaneous injection over the calvarium of a mouse. This assay allows testing of the effect of substances on both bone resorption and bone formation in a relatively simple in vivo model. The analysis is carried out by histological means, usually in glycolmethacrylate-embedded tissue, allowing for histochemical analysis and for a variety of different histological staining methods which are also described in detail.
UR - http://www.scopus.com/inward/record.url?scp=84555187800&partnerID=8YFLogxK
U2 - 10.1007/978-1-61779-415-5_33
DO - 10.1007/978-1-61779-415-5_33
M3 - Article
C2 - 22130950
VL - 816
SP - 537
EP - 544
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -