The chaperone FACT and histone H2B ubiquitination maintain S. pombe genome architecture through genic and subtelomeric functions

Magdalena Murawska, Tamas Schauer, Atsushi Matsuda, Marcus Wilson, Thomas Pysik, Felix Wojcik, Tom W. Muir, Yasushi Hiraoka, Tobias Straub, Andreas G. Ladurner

Research output: Contribution to journalArticlepeer-review

Abstract

The histone chaperone FACT and histone H2B ubiquitination (H2Bub) facilitate RNA Polymerase II (RNAPII) passage through chromatin, yet it is not clear how they cooperate mechanistically. We used genomics, genetic, biochemical and microscopic approaches to dissect their interplay in Schizosaccharomyces pombe. We show that FACT and H2Bub globally repress antisense transcripts near the 5’ end of genes and inside gene bodies, respectively. The accumulation of these transcripts is accompanied by changes at genic nucleosomes and RNAPII redistribution. H2Bub is required for FACT activity in genic regions. In the H2Bub mutant, FACT binding to chromatin is altered and its association with histones is stabilized, which leads to the reduction of genic nucleosomes. Interestingly, FACT depletion globally restores nucleosomes in the H2Bub mutant. Moreover, in the absence of Pob3, the FACT Spt16 subunit controls the 3’ end of genes. Furthermore, FACT maintains nucleosomes in subtelomeric regions, which is crucial for their compaction.
Original languageEnglish
Pages (from-to)501-513.e7
JournalMolecular Cell
Volume77
Issue number3
DOIs
Publication statusPublished - 11 Dec 2019

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