The chaperonin GroEL and other heat-shock proteins, besides DnaK, participate in ribosome biogenesis in Escherichia coli

Aziz El Hage, Miriam Sbai, Jean-Herve Alix

Research output: Contribution to journalArticlepeer-review

Abstract

It has been shown that in Escherichia coli the chaperone DnaK is necessary for the late stages of 50S and 30S ribosomal subunit assembly in vivo. Here we focus on the roles of other HSPs (heat-shock proteins), including the chaperonin GroEL, in addition to DnaK, in ribosome biogenesis at high temperature. GroEL is shown to be required for the very late 45S-->50S step in the biogenesis of the large ribosome subunit, but not for 30S assembly. Interestingly, overproduction of GroES/GroEL can partially compensate for a lack of DnaK/DnaJ at 44 degrees C.
Original languageEnglish
Pages (from-to)796-808
Number of pages13
JournalMolecular genetics and genomics
Volume264
Issue number6
DOIs
Publication statusPublished - 1 Feb 2001

Keywords

  • DNAK/J chaperones
  • GroEL/ES chaperonins
  • ribosome biogenesis
  • heat shock proteins
  • Escherichia coli

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