The clinical significance of the detection of hepatitis GBV-C RNA in the serum of patients with fulminant, presumed viral, hepatitis

G H Haydon, L M Jarvis, K J Simpson, P C Hayes, P Simmonds

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

In a significant number of cases of fulminant (presumed viral) hepatitis worldwide, no aetiological agent has been identified. Recently, it has been suggested that a newly described flavivirus, GBV-C, is responsible for some of these cases. This study aimed to assess the clinical significance of GBV-C RNA, demonstrated by reverse transcriptase-polymerase chain reaction (RT-PCR), in the serum of patients with fulminant non-A to E hepatitis. Twenty-three consecutive cases of non-A to E fulminant hepatitis were included in the study. GBV-C RNA was reverse transcribed and amplified using two RT-PCR based detection methods. Medical records were examined to assess clinical history, duration and mode of infection, transfusion history, liver histology and clinical outcome. Five (three female, two male; mean age 21.2 years) of 23 patients had GBV-C RNA detected in their serum by RT-PCR: all five patients were RT-PCR positive following amplification by primers specific for the 5' non-coding region (NCR), whilst four were positive by primers for the NS3 region. Prior to the onset of illness, two patients had risk factors for transmission of an infectious agent; however, all five patients had been transfused during their illness, prior to testing for GBV-C. Of these, two (of two in whom serum was available) were negative for GBV-C after the onset of fulminant hepatitis but before their first transfusion. This study does not support the hypothesis that the detection of hepatitis G virus (HGV)/GBV-C RNA in the serum of patients with fulminant hepatitis indicates a causal association. However, it does demonstrate that a careful transfusion history and screening of blood products is vital before the importance of GBV-C in the aetiology of fulminant hepatitis can be established.
Original languageEnglish
Pages (from-to)45-9
Number of pages5
JournalJournal of Viral Hepatitis
Issue number1
Publication statusPublished - Jan 1997

Keywords / Materials (for Non-textual outputs)

  • Adolescent
  • Adult
  • Blood Transfusion
  • Child
  • Female
  • Flaviviridae
  • Hepatic Encephalopathy
  • Hepatitis, Viral, Human
  • Humans
  • Male
  • Polymerase Chain Reaction
  • RNA, Viral
  • Transcription, Genetic


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