The colony-stimulating factor 1 receptor is expressed on dendritic cells during differentiation and regulates their expansion

Kelli P A MacDonald, Vanessa Rowe, Helen M Bofinger, Ranjeny Thomas, Tedjo Sasmono, David A Hume, Geoffrey R Hill

Research output: Contribution to journalArticlepeer-review

Abstract

The lineage of dendritic cells (DC), and in particular their relationship to monocytes and macrophages, remains obscure. Furthermore, the requirement for the macrophage growth factor CSF-1 during DC homeostasis is unclear. Using a transgenic mouse in which the promoter for the CSF-1R (c-fms) directs the expression of enhanced GFP in cells of the myeloid lineage, we determined that although the c-fms promoter is inactive in DC precursors, it is up-regulated in all DC subsets during differentiation. Furthermore, plasmacytoid DC and all CD11c(high) DC subsets are reduced by 50-70% in CSF-1-deficient osteopetrotic mice, confirming that CSF-1 signaling is required for the optimal differentiation of DC in vivo. These data provide additional evidence that the majority of tissue DC is of myeloid origin during steady state and supports a close relationship between DC and macrophage biology in vivo.
Original languageEnglish
Pages (from-to)1399-405
Number of pages7
JournalThe Journal of Immunology
Volume175
Issue number3
Publication statusPublished - 1 Aug 2005

Keywords

  • Animals
  • Antigens, CD11c
  • Antigens, CD45
  • Blood Cell Count
  • Cell Differentiation
  • Cell Proliferation
  • Cells, Cultured
  • Dendritic Cells
  • Green Fluorescent Proteins
  • Hematopoietic Stem Cells
  • Macrophage Colony-Stimulating Factor
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Mice, Knockout
  • Mice, Transgenic
  • Receptor, Macrophage Colony-Stimulating Factor
  • Signal Transduction

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