The conserved metalloprotease invadolysin localizes to the surface of lipid droplets

Neville Cobbe, Kathryn M. Marshall, Shubha Gururaja Rao, Ching-Wen Chang, Francesca Di Cara, Edward Duca, Sharron Vass, Adam Kassan, Margarete M. S. Heck

Research output: Contribution to journalArticlepeer-review

Abstract

Invadolysin is a metalloprotease conserved in many different organisms, previously shown to be essential in Drosophila with roles in cell division and cell migration. The gene seems to be ubiquitously expressed and four distinct splice variants have been identified in human cells but not in most other species examined. Immunofluorescent detection of human invadolysin in cultured cells reveals the protein to be associated with the surface of lipid droplets. By means of subcellular fractionation, we have independently confirmed the association of invadolysin with lipid droplets. We thus identify invadolysin as the first metalloprotease located on these dynamic organelles. In addition, analysis of larval fat-body morphological appearance and triglyceride levels in the Drosophila invadolysin mutant suggests that invadolysin plays a role in lipid storage or metabolism.

Original languageEnglish
Pages (from-to)3414-3423
Number of pages10
JournalJournal of Cell Science
Volume122
Issue number18
DOIs
Publication statusPublished - 15 Sep 2009

Keywords

  • Invadolysin
  • Lipid droplets
  • Metalloprotease
  • Phylogeny

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