The correlation between plasminogen activator activity and thymidine incorporation in mouse bone marrow-derived macrophages. Opposing actions of colony-stimulating factor, phorbol myristate acetate, dexamethasone and prostaglandin E

D A Hume, S Gordon

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Plasminogen activator activity and [3H]thymidine incorporation were studied in mouse bone marrow-derived macrophages. The two activities correlated closely in the presence of stimulatory (colony-stimulating factor, phorbol myristate acetate, PMA) and inhibitory (dexamethasone, prostaglandin E1) signals. The actions of dexamethasone and prostaglandin E1 could be overcome by either stimulatory agent, so that the net effect was an alteration in sensitivity of the macrophages to colony-stimulating factor, or PMA. The sensitivity of bone marrow-derived macrophages to CSF-1 was also reduced by the addition of small numbers of CSF-1 unresponsive peritoneal macrophages. Plasminogen activator induction was not a sufficient signal for [3H]thymidine incorporation which requires an additional macromolecular serum component. The serum component was found not to be plasminogen.
Original languageEnglish
Pages (from-to)347-55
Number of pages9
JournalExperimental Cell Research
Volume150
Issue number2
DOIs
Publication statusPublished - Feb 1984

Keywords / Materials (for Non-textual outputs)

  • Alprostadil
  • Animals
  • Ascitic Fluid
  • Bone Marrow Cells
  • Cell Division
  • Cells, Cultured
  • Colony-Stimulating Factors
  • Dexamethasone
  • Macrophages
  • Mice
  • Plasminogen Activators
  • Prostaglandins E
  • Tetradecanoylphorbol Acetate
  • Thymidine

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