The cyclic dipeptide CI-4 [cyclo-(L-Arg-D-Pro)] inhibits family 18 chitinases by structural mimicry of a reaction intermediate

Douglas R. Houston, Ian Eggleston, Bjørnar Synstad, Vincent G.H. Eijsink, Daan M.F. Van Aalten*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Family 18 chitinases are attractive targets for the development of new inhibitors with chemotherapeutic potential against fungi, insects and protozoan/nematodal parasites. Although several inhibitors have been identified, these are based on complex chemistry, which hampers iterative structure-based optimization. Here we report the details of chitinase inhibition by the natural product peptide CI-4 [cyclo-(L-Arg-D-Pro)], which possesses activity against the human pathogenic fungus Candida albicans, and describe a 1.7 Å (0.17 nm) crystal structure of CI-4 in complex with the enzyme. The structure reveals that the cyclic dipeptide inhibits chitinases by structurally mimicking a reaction intermediate, and could, on the basis of its accessible chemistry, be a candidate for further optimization.

Original languageEnglish
Pages (from-to)23-27
Number of pages5
JournalBiochemical Journal
Volume368
Issue number1
DOIs
Publication statusPublished - 15 Nov 2002

Keywords

  • Allosamidin
  • Candida albicans
  • Glycoside hydrolase
  • Plasmodium falciparum
  • X-ray crystallography

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