The design of microfluidic affinity chromatography systems for the separation of bioanalytes

Daniel Friedrich, Colin P Please, Tracy Melvin

Research output: Contribution to journalArticlepeer-review

Abstract

The analytical (numerical) design of planar microfluidic affinity chromatography devices, which consist of multiple separation lanes and multiple, different surface-immobilised receptor patterns in each lane, is
described. The model is based on the analytical solution of the transport-reaction equations in microfluidic systems of low Gratz number and for injection of small analyte plugs. The results reveal a simple approach for the design of microfluidic affinity chromatography devices tailored to the separation of
bioanalytes, where receptors with high binding affinity are available. These devices have been designed for bioanalytical applications in mind, most notably for the proteomics field; the results are illustrated with an example using beta-Amyloid binding peptides.
Original languageEnglish
Pages (from-to)163-171
JournalJournal of Chromatography B
Volume910
DOIs
Publication statusPublished - 2012

Keywords

  • Affinity chromatography; Microfluidic; mathematical modelling; separation; design strategy; beta-Amyloid binding peptides

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