The developmental dismantling of pluripotency is reversed by ectopic Oct4 expression

Rodrigo Osorno, Anestis Tsakiridis, Frederick Wong, Noemí Cambray, Constantinos Economou, Ronald Wilkie, Guillaume Blin, Paul J Scotting, Ian Chambers, Valerie Wilson

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The transcription factors Nanog and Oct4 regulate pluripotency in the pre-implantation epiblast and in derivative embryonic stem cells. During post-implantation development, the precise timing and mechanism of the loss of pluripotency is unknown. Here, we show that in the mouse, pluripotency is extinguished at the onset of somitogenesis, coincident with reduced expression and chromatin accessibility of Oct4 and Nanog regulatory regions. Prior to somitogenesis expression of both Nanog and Oct4 is regionalized. We show that pluripotency tracks the in vivo level of Oct4 and not Nanog by assessing the ability to reactivate or maintain Nanog expression in cell culture. Enforced Oct4 expression in somitogenesis-stage tissue provokes rapid reopening of Oct4 and Nanog chromatin, Nanog re-expression and resuscitates moribund pluripotency. Our data suggest that decreasing Oct4 expression is converted to a sudden drop in competence to maintain pluripotency gene regulatory network activity that is subsequently stabilized by epigenetic locks.
Original languageEnglish
Pages (from-to)2288-2298
Number of pages11
Issue number13
Publication statusPublished - Jul 2012

Keywords / Materials (for Non-textual outputs)

  • Pluripotency
  • Mouse embryo
  • Oct4
  • Nanog
  • Teratocarcinoma
  • Transcription factor
  • Chromatin
  • Somitogenesis


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