The differential expression of Kiss1, MMP9 and angiogenic regulators across the feto-maternal interface of healthy human pregnancies: implications for trophoblast invasion and vessel development

Mushi Matjila; Robert Millar; Zephne van der Spuy; Arieh Katz

doi:10.1371/journal.pone.0063574

The differential expression of Kiss1, MMP9 and angiogenic regulators across the feto-maternal interface of healthy human pregnancies: implications for trophoblast invasion and vessel development

Mushi Matjila, Robert Millar, Zephne van der Spuy, Arieh Katz

Deanery of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Genes involved in invasion of trophoblast cells and angiogenesis are crucial in determining pregnancy outcome. We therefore studied expression profiles of these genes in both fetal and maternal tissues to enhance our understanding of feto-maternal dialogue. We investigated the expression of genes involved in trophoblast invasion, namely Kiss1, Kiss1 Receptor (Kiss1R) and MMP9 as well as the expression of angiogenic ligands Vascular Endothelial Growth Factor-A (VEGF-A) and Prokineticin-1 (PROK1) and their respective receptors (VEGFR1, VEGFR2 and PROK1R) across the feto-maternal interface of healthy human pregnancies. The placenta, placental bed and decidua parietalis were sampled at elective caesarean delivery. Real-time RT-PCR was used to investigate transcription, while immunohistochemistry and western blot analyses were utilized to study protein expression. We found that the expression of Kiss1 (p

Original language	English
Pages (from-to)	e63574
Journal	PLoS ONE
Volume	8
Issue number	5
DOIs	https://doi.org/10.1371/journal.pone.0063574
Publication status	Published - 16 May 2013

Keywords

Female
Gastrointestinal Hormones
Humans
Immunohistochemistry
Kisspeptins
Matrix Metalloproteinase 9
Placenta
Pregnancy
Receptors, G-Protein-Coupled
Trophoblasts
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factor Receptor-1
Vascular Endothelial Growth Factor Receptor-2
Vascular Endothelial Growth Factor, Endocrine-Gland-Derived

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10.1371/journal.pone.0063574

The differential expression of Kiss1, MMP9 and angiogenic regulators across the feto-maternal interface of healthy human pregnancies
Copyright: © 2013 Matjila et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Final published version, 9.76 MBLicence: Creative Commons: Attribution (CC-BY)

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title = "The differential expression of Kiss1, MMP9 and angiogenic regulators across the feto-maternal interface of healthy human pregnancies: implications for trophoblast invasion and vessel development",

abstract = "Genes involved in invasion of trophoblast cells and angiogenesis are crucial in determining pregnancy outcome. We therefore studied expression profiles of these genes in both fetal and maternal tissues to enhance our understanding of feto-maternal dialogue. We investigated the expression of genes involved in trophoblast invasion, namely Kiss1, Kiss1 Receptor (Kiss1R) and MMP9 as well as the expression of angiogenic ligands Vascular Endothelial Growth Factor-A (VEGF-A) and Prokineticin-1 (PROK1) and their respective receptors (VEGFR1, VEGFR2 and PROK1R) across the feto-maternal interface of healthy human pregnancies. The placenta, placental bed and decidua parietalis were sampled at elective caesarean delivery. Real-time RT-PCR was used to investigate transcription, while immunohistochemistry and western blot analyses were utilized to study protein expression. We found that the expression of Kiss1 (p",

keywords = "Female, Gastrointestinal Hormones, Humans, Immunohistochemistry, Kisspeptins, Matrix Metalloproteinase 9, Placenta, Pregnancy, Receptors, G-Protein-Coupled, Trophoblasts, Vascular Endothelial Growth Factor A, Vascular Endothelial Growth Factor Receptor-1, Vascular Endothelial Growth Factor Receptor-2, Vascular Endothelial Growth Factor, Endocrine-Gland-Derived",

author = "Mushi Matjila and Robert Millar and {van der Spuy}, Zephne and Arieh Katz",

note = "the MRC (UK) supported the studies at the MRC Human Reproductive Sciences Unit (HRSU) in Edinburgh",

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The differential expression of Kiss1, MMP9 and angiogenic regulators across the feto-maternal interface of healthy human pregnancies : implications for trophoblast invasion and vessel development. / Matjila, Mushi; Millar, Robert; van der Spuy, Zephne; Katz, Arieh.

In: PLoS ONE, Vol. 8, No. 5, 16.05.2013, p. e63574.

Research output: Contribution to journal › Article › peer-review

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