The differential expression of Kiss1, MMP9 and angiogenic regulators across the feto-maternal interface of healthy human pregnancies: implications for trophoblast invasion and vessel development

Mushi Matjila, Robert Millar, Zephne van der Spuy, Arieh Katz

Research output: Contribution to journalArticlepeer-review

Abstract

Genes involved in invasion of trophoblast cells and angiogenesis are crucial in determining pregnancy outcome. We therefore studied expression profiles of these genes in both fetal and maternal tissues to enhance our understanding of feto-maternal dialogue. We investigated the expression of genes involved in trophoblast invasion, namely Kiss1, Kiss1 Receptor (Kiss1R) and MMP9 as well as the expression of angiogenic ligands Vascular Endothelial Growth Factor-A (VEGF-A) and Prokineticin-1 (PROK1) and their respective receptors (VEGFR1, VEGFR2 and PROK1R) across the feto-maternal interface of healthy human pregnancies. The placenta, placental bed and decidua parietalis were sampled at elective caesarean delivery. Real-time RT-PCR was used to investigate transcription, while immunohistochemistry and western blot analyses were utilized to study protein expression. We found that the expression of Kiss1 (p
Original languageEnglish
Pages (from-to)e63574
JournalPLoS ONE
Volume8
Issue number5
DOIs
Publication statusPublished - 16 May 2013

Keywords

  • Female
  • Gastrointestinal Hormones
  • Humans
  • Immunohistochemistry
  • Kisspeptins
  • Matrix Metalloproteinase 9
  • Placenta
  • Pregnancy
  • Receptors, G-Protein-Coupled
  • Trophoblasts
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factor Receptor-1
  • Vascular Endothelial Growth Factor Receptor-2
  • Vascular Endothelial Growth Factor, Endocrine-Gland-Derived

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