The Drosophila RGS protein Loco is required for dorsal/ventral axis formation of the egg and embryo, and nurse cell dumping

Stephen Pathirana, Debiao Zhao, Mary Bownes

Research output: Contribution to journalArticlepeer-review

Abstract

The loco gene encodes members of a family of RGS proteins responsible for the negative regulation of G-protein signalling. At least two transcripts of loco are expressed in oogenesis, loco-c2 is observed in the anterior-dorsal follicle cells and is downstream of the epidermal growth factor receptor signalling pathway, initiated in the oocyte. loco-c3 is a new transcript of loco, which is expressed in the nurse cells from stage 6 onwards. Analysis of newly generated mutants and antisense technology enabled us to establish that disrupting loco in follicle cells results in ventralized eggs, while disrupting loco in nurse cells results in short eggs, due to defective dumping of the nurse cell cytoplasm into the oocyte.
Original languageEnglish
Pages (from-to)137-50
Number of pages14
JournalMechanisms of Development
Volume109
Issue number2
DOIs
Publication statusPublished - Dec 2001

Keywords

  • Amino Acid Sequence
  • Animals
  • Blotting, Western
  • Chromosome Mapping
  • Cytoplasm/metabolism
  • DNA, Complementary/metabolism
  • Drosophila
  • Drosophila Proteins
  • Embryo, Nonmammalian/physiology
  • Female
  • Gene Library
  • In Situ Hybridization
  • Insect Proteins/metabolism
  • Male
  • Models, Genetic
  • Molecular Sequence Data
  • Mutation
  • Nerve Tissue Proteins/chemistry
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/physiology
  • Oligonucleotides, Antisense/pharmacology
  • Oogenesis
  • Ovum/physiology
  • Phenotype
  • Protein Kinases
  • RGS Proteins/chemistry
  • RGS Proteins/physiology
  • RNA/metabolism
  • RNA, Messenger/metabolism
  • Receptor, Epidermal Growth Factor/metabolism
  • Receptors, Invertebrate Peptide/metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Signal Transduction
  • Time Factors
  • Tissue Distribution
  • Transformation, Genetic
  • Transforming Growth Factor alpha
  • Transforming Growth Factors/metabolism

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