The E3 ubiquitin ligase activity of Ring1B is not essential for early mouse development

Robert S. Illingworth, Michael Moffat, Abigail Mann, David Read, Chris Hunter, Madapura M Pradeepa, Ian R. Adams, Wendy A. Bickmore

Research output: Contribution to journal › Article › peer-review

Abstract

Polycomb-repressive complex 1 (PRC1) and PRC2 maintain repression at many developmental genes in mouse embryonic stem cells and are required for early development. However, it is still unclear how they are targeted and how they function. We show that the ability of RING1B, a core component of PRC1, to ubiquitinate histone H2A is dispensable for early mouse embryonic development and much of the gene repression activity of PRC1. Our data support a model in which PRC1 and PRC2 reinforce each other's binding but suggest that the key functions of PRC1 lie beyond the enzymatic capabilities of RING1B.
Original languageEnglish
Pages (from-to)1897-1902
Number of pages6
JournalGenes & Development
Volume29
Issue number18
DOIs
Publication statusPublished - 15 Sept 2015

Keywords / Materials (for Non-textual outputs)

  • PRC1
  • histone ubiquitination
  • H3K27me3
  • embryonic stem cell
  • Polycomb

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    • The role of spatial nuclear organisation in genome function

      Bickmore, W. (Principal Investigator)

      1/04/12 → 31/03/18

      Project: Research

    • CROATIA PROGRAMME

      Wright, A. (Principal Investigator), Adams, I. (Co-investigator), Aligianis, I. (Co-investigator), Baldock, R. (Co-investigator), Bickmore, W. (Co-investigator), Caceres, J. (Co-investigator), Dorin, J. (Co-investigator), Dunlop, M. (Co-investigator), FitzPatrick, D. (Co-investigator), Haley, C. (Co-investigator), Hastie, N. (Co-investigator), Hill, B. (Co-investigator), Jackson, I. (Co-investigator), Jackson, A. (Co-investigator), Kudla, G. (Co-investigator), Meehan, R. (Co-investigator), O'Connell, M. (Co-investigator), Overton, I. (Co-investigator), Patton, E. (Co-investigator), Taylor, M. (Co-investigator), Tenesa, A. (Co-investigator) & Van Heyningen, V. (Co-investigator)

      MRC

      1/04/12 → 31/07/13

      Project: Research

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