The E3 ubiquitin ligase activity of Ring1B is not essential for early mouse development

Robert S. Illingworth; Michael Moffat; Abigail Mann; David  Read; Chris Hunter; Madapura M Pradeepa; Ian R. Adams; Wendy A. Bickmore

doi:10.1101/gad.268151.115

The E3 ubiquitin ligase activity of Ring1B is not essential for early mouse development

Robert S. Illingworth, Michael Moffat, Abigail Mann, David Read, Chris Hunter, Madapura M Pradeepa, Ian R. Adams, Wendy A. Bickmore

Research output: Contribution to journal › Article › peer-review

Abstract

Polycomb-repressive complex 1 (PRC1) and PRC2 maintain repression at many developmental genes in mouse embryonic stem cells and are required for early development. However, it is still unclear how they are targeted and how they function. We show that the ability of RING1B, a core component of PRC1, to ubiquitinate histone H2A is dispensable for early mouse embryonic development and much of the gene repression activity of PRC1. Our data support a model in which PRC1 and PRC2 reinforce each other's binding but suggest that the key functions of PRC1 lie beyond the enzymatic capabilities of RING1B.

Original language	English
Pages (from-to)	1897-1902
Number of pages	6
Journal	Genes & Development
Volume	29
Issue number	18
DOIs	https://doi.org/10.1101/gad.268151.115
Publication status	Published - 15 Sep 2015

Keywords

PRC1
histone ubiquitination
H3K27me3
embryonic stem cell
Polycomb

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10.1101/gad.268151.115Licence: Creative Commons: Attribution (CC-BY)

The E3 ubiquitin ligase activity of Ring1B is not essential for early mouse development_Rob Illingworth A
This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.
Final published version, 966 KBLicence: Creative Commons: Attribution (CC-BY)

The role of spatial nuclear organisation in genome function
Bickmore, W.
1/04/12 → 31/03/18
Project: Research
CROATIA PROGRAMME
Wright, A., Adams, I., Aligianis, I., Baldock, R., Bickmore, W., Caceres, J., Dorin, J., Dunlop, M., FitzPatrick, D., Haley, C., Hastie, N., Hill, B., Jackson, I., Jackson, A., Kudla, G., Meehan, R., O'Connell, M., Overton, I., Patton, E., Taylor, M., Tenesa, A. & Van Heyningen, V.
MRC
1/04/12 → 31/07/13
Project: Research

Ian Adams
- Ian.Adamsigmm.ed.acuk
- Deanery of Molecular, Genetic and Population Health Sciences - Personal Chair of Germline Biology
- MRC Human Genetics Unit
Person: Academic: Research Active
Wendy Bickmore
- Deanery of Molecular, Genetic and Population Health Sciences - Director of Human Genetics Unit
- MRC Human Genetics Unit
Person: Academic: Research Active

Cite this

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title = "The E3 ubiquitin ligase activity of Ring1B is not essential for early mouse development",

abstract = "Polycomb-repressive complex 1 (PRC1) and PRC2 maintain repression at many developmental genes in mouse embryonic stem cells and are required for early development. However, it is still unclear how they are targeted and how they function. We show that the ability of RING1B, a core component of PRC1, to ubiquitinate histone H2A is dispensable for early mouse embryonic development and much of the gene repression activity of PRC1. Our data support a model in which PRC1 and PRC2 reinforce each other's binding but suggest that the key functions of PRC1 lie beyond the enzymatic capabilities of RING1B.",

keywords = "PRC1, histone ubiquitination, H3K27me3, embryonic stem cell, Polycomb",

author = "Illingworth, {Robert S.} and Michael Moffat and Abigail Mann and David Read and Chris Hunter and Pradeepa, {Madapura M} and Adams, {Ian R.} and Bickmore, {Wendy A.}",

year = "2015",

month = sep,

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doi = "10.1101/gad.268151.115",

language = "English",

volume = "29",

pages = "1897--1902",

journal = "Genes and Development",

issn = "0890-9369",

publisher = "Cold Spring Harbor Laboratory Press",

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AU - Illingworth, Robert S.

AU - Moffat, Michael

AU - Mann, Abigail

AU - Read, David

AU - Hunter, Chris

AU - Pradeepa, Madapura M

AU - Adams, Ian R.

AU - Bickmore, Wendy A.

PY - 2015/9/15

Y1 - 2015/9/15

N2 - Polycomb-repressive complex 1 (PRC1) and PRC2 maintain repression at many developmental genes in mouse embryonic stem cells and are required for early development. However, it is still unclear how they are targeted and how they function. We show that the ability of RING1B, a core component of PRC1, to ubiquitinate histone H2A is dispensable for early mouse embryonic development and much of the gene repression activity of PRC1. Our data support a model in which PRC1 and PRC2 reinforce each other's binding but suggest that the key functions of PRC1 lie beyond the enzymatic capabilities of RING1B.

AB - Polycomb-repressive complex 1 (PRC1) and PRC2 maintain repression at many developmental genes in mouse embryonic stem cells and are required for early development. However, it is still unclear how they are targeted and how they function. We show that the ability of RING1B, a core component of PRC1, to ubiquitinate histone H2A is dispensable for early mouse embryonic development and much of the gene repression activity of PRC1. Our data support a model in which PRC1 and PRC2 reinforce each other's binding but suggest that the key functions of PRC1 lie beyond the enzymatic capabilities of RING1B.

KW - PRC1

KW - histone ubiquitination

KW - H3K27me3

KW - embryonic stem cell

KW - Polycomb

U2 - 10.1101/gad.268151.115

DO - 10.1101/gad.268151.115

M3 - Article

VL - 29

SP - 1897

EP - 1902

JO - Genes and Development

JF - Genes and Development

SN - 0890-9369

IS - 18

ER -

The E3 ubiquitin ligase activity of Ring1B is not essential for early mouse development

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Keywords

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The role of spatial nuclear organisation in genome function

CROATIA PROGRAMME

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Wendy Bickmore

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