The effect of omega-3 polyunsaturated fatty acids on the inflammatory response of the amnion

Lorraine Frew, N.U. Sugiarto, Shalini P. Rajagopal, J He, Rosemary Leask, Jane E Norman, Simon C Riley, Sarah J Stock

Research output: Contribution to journalArticlepeer-review

Abstract

Objective
To investigate the effect of omega-3 PUFAs, eicosapentanoic acid (EPA) and docosohexanoic acid (DHA) on inflammatory cytokine production in the amnion.

Study design
Amnion explants were obtained at elective caesarean sections and cultured in vitro with EPA and DHA. IL-8 and IL-6 secretion was determined by ELISA, the role of PPARγ was investigated using specific agonists and antagonists and activity of MMP assessed by gelatin zymography.

Results
A combination of EPA and DHA significantly reduced the concentration of IL-8 and IL-6 released into the supernatant compared to untreated controls (p<0.001). Stimulation of PPARγ with troglitazone reduced IL-8 production, and the PPARγ antagonist GW9662 partially reversed this effect. The activity of MMP-9 was also significantly reduced by treatment with EPA and DHA in combination compared to untreated control (p<0.05).

Conclusion
The omega-3 PUFAs EPA and DHA decrease the inflammatory response of the amnion, and this may be partially mediated through PPARγ.
Original languageEnglish
Pages (from-to)221-225
Number of pages5
JournalProstaglandins, Leukotrienes and Essential Fatty Acids
Volume89
Issue number4
Early online date23 Jul 2013
DOIs
Publication statusPublished - Sep 2013

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