TY - JOUR
T1 - The effect of polymorphisms in tumor necrosis factor-alpha, interleukin-10, and transforming growth factor-beta 1 genes in acute hepatic allograft rejection
AU - Unit, Scottish Liver
AU - Pravica, Vera
AU - Perrey, C
AU - Unit, Scottish Liver
AU - Plevris, JN
AU - Unit, Scottish Liver
AU - Hutchinson, Jonathan P.
PY - 2000/4/15
Y1 - 2000/4/15
N2 - Background. The occurrence of acute rejection in orthotopic liver transplantation is unpredictable, The role of cytokines in the process of rejection is not entirely clear. We investigated polymorphisms in the genes encoding tumor necrosis factor (TNF)-alpha, interleukin (IL)-10, and transforming growth factor (TGF)-beta 1, which affect the amount of cytokine produced in vitro, in a liver transplant population to determine any association with acute rejection.Method. DNA was extracted from whole blood of liver transplant patients. After amplification with polymerase chain reactions, the polymorphisms at TNF-alpha -308, IL-10 -1082, and TGF-beta 1 +869 and +915 were determined using sequence-specific oligonucleotide probes. Acute cellular rejection was a clinical and histological diagnosis.Results. Acute cellular rejection requiring treatment occurred in 68 (48%) of 144 patients. Acute cellular rejection was significantly associated with the TNF-alpha -308 A/A genotype (PConclusion. Patients with a homozygous TNF-alpha -308 genotype A/A are more likely to suffer from acute cellular rejection after liver transplantation.
AB - Background. The occurrence of acute rejection in orthotopic liver transplantation is unpredictable, The role of cytokines in the process of rejection is not entirely clear. We investigated polymorphisms in the genes encoding tumor necrosis factor (TNF)-alpha, interleukin (IL)-10, and transforming growth factor (TGF)-beta 1, which affect the amount of cytokine produced in vitro, in a liver transplant population to determine any association with acute rejection.Method. DNA was extracted from whole blood of liver transplant patients. After amplification with polymerase chain reactions, the polymorphisms at TNF-alpha -308, IL-10 -1082, and TGF-beta 1 +869 and +915 were determined using sequence-specific oligonucleotide probes. Acute cellular rejection was a clinical and histological diagnosis.Results. Acute cellular rejection requiring treatment occurred in 68 (48%) of 144 patients. Acute cellular rejection was significantly associated with the TNF-alpha -308 A/A genotype (PConclusion. Patients with a homozygous TNF-alpha -308 genotype A/A are more likely to suffer from acute cellular rejection after liver transplantation.
KW - LIVER-TRANSPLANTATION
KW - MESSENGER-RNA
KW - GROWTH-FACTOR-BETA-1
KW - EXPRESSION
KW - CORRELATE
KW - PROMOTER
UR - https://journals.lww.com/transplantjournal/Fulltext/2000/04150/THE_EFFECT_OF_POLYMORPHISMS_IN_TUMOR_NECROSIS.54.aspx
M3 - Article
SN - 0041-1337
VL - 69
SP - 1514
EP - 1517
JO - Transplantation
JF - Transplantation
IS - 7
ER -