The effects of Arcanobacterium pyogenes on endometrial function in vitro, and on uterine and ovarian function in vivo

A. N.A. Miller, E. J. Williams, K. Sibley, S. Herath, E. A. Lane, J. Fishwick, D. M. Nash, A. N. Rycroft, H. Dobson, C. E. Bryant, I. M. Sheldon*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Uterine bacterial infection after parturition causes endometritis, perturbs ovarian function and leads to infertility in cattle. Although endometritis is caused by mixed infections, endometrial pathology is associated with the presence of Arcanobacterium pyogenes. The aims of the present study were to determine the effects of A. pyogenes on endometrial function in vitro, and on uterine and ovarian function in vivo. Heat-killed A. pyogenes did not affect the production of prostaglandin F (PGF) or prostaglandin E2 (PGE) from endometrial explants, or purified populations of endometrial epithelial or stromal cells. However, the explants produced more PGF and PGE than controls when treated with a bacteria-free filtrate (BFF) cultured from A. pyogenes. Similarly, BFF stimulated PGF and PGE production by epithelial and stromal cells, respectively. So, BFF or control PBS was infused into the uterus of heifers (n = 7 per group) for 8 days, starting the day after estrus. Emergence of the follicle wave, dominant follicle or corpus luteum diameter, and peripheral plasma FSH, LH, estradiol, progesterone, PGFM, or acute phase protein concentrations were unaffected by the BFF infusion. In the live animal it is likely that the intact uterine mucosa limits the exposure of the endometrial cells to the exotoxin of A. pyogenes, whereas the cells are readily exposed to the toxin in vitro.

Original languageEnglish
Pages (from-to)972-980
Number of pages9
JournalTheriogenology
Volume68
Issue number7
Early online date7 Sept 2007
DOIs
Publication statusPublished - 15 Oct 2007

Keywords / Materials (for Non-textual outputs)

  • Inflammation
  • Postpartum infection
  • Reproductive immunology

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