The effects of serum from patients with acute liver failure on the growth and metabolism of Hep G2 cells

Q. Shi; J. D.S. Gaylor; R. Cousins; J. Plevris; P. C. Hayes; M. H. Grant

doi:10.1046/j.1525-1594.1998.06211.x

The effects of serum from patients with acute liver failure on the growth and metabolism of Hep G2 cells

Q. Shi, J. D.S. Gaylor, R. Cousins, J. Plevris, P. C. Hayes, M. H. Grant^*

^*Corresponding author for this work

School of Mathematics

Research output: Contribution to journal › Article › peer-review

Abstract

In many bioartificial liver systems currently being designed and evaluated for use in fulminant hepatic failure, direct contact is required between the patient's blood and the liver cells in the device. The efficacy of such devices will be influenced by the interaction of fulminant hepatic failure (FHF) patient serum with the cells. We have found that FHF serum inhibits the growth rate and the synthesis of DNA, RNA, and protein; disturbs glutathione homeostasis; and induces morphological changes in cultured human Hep G2 cells. These interactions should influence the design of bioartificial liver devices based on proliferating cell lines and indicate the requirement to pretreat FHF patient plasma to reduce the toxin load.

Original language	English
Pages (from-to)	1023-1030
Number of pages	8
Journal	Artificial organs
Volume	22
Issue number	12
DOIs	https://doi.org/10.1046/j.1525-1594.1998.06211.x
Publication status	Published - 1998

Keywords

Bioartificial liver
Cytotoxicity of fulminant hepatic failure serum
Hep G2 cells

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10.1046/j.1525-1594.1998.06211.x

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abstract = "In many bioartificial liver systems currently being designed and evaluated for use in fulminant hepatic failure, direct contact is required between the patient's blood and the liver cells in the device. The efficacy of such devices will be influenced by the interaction of fulminant hepatic failure (FHF) patient serum with the cells. We have found that FHF serum inhibits the growth rate and the synthesis of DNA, RNA, and protein; disturbs glutathione homeostasis; and induces morphological changes in cultured human Hep G2 cells. These interactions should influence the design of bioartificial liver devices based on proliferating cell lines and indicate the requirement to pretreat FHF patient plasma to reduce the toxin load.",

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AU - Shi, Q.

AU - Gaylor, J. D.S.

AU - Cousins, R.

AU - Plevris, J.

AU - Hayes, P. C.

AU - Grant, M. H.

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N2 - In many bioartificial liver systems currently being designed and evaluated for use in fulminant hepatic failure, direct contact is required between the patient's blood and the liver cells in the device. The efficacy of such devices will be influenced by the interaction of fulminant hepatic failure (FHF) patient serum with the cells. We have found that FHF serum inhibits the growth rate and the synthesis of DNA, RNA, and protein; disturbs glutathione homeostasis; and induces morphological changes in cultured human Hep G2 cells. These interactions should influence the design of bioartificial liver devices based on proliferating cell lines and indicate the requirement to pretreat FHF patient plasma to reduce the toxin load.

AB - In many bioartificial liver systems currently being designed and evaluated for use in fulminant hepatic failure, direct contact is required between the patient's blood and the liver cells in the device. The efficacy of such devices will be influenced by the interaction of fulminant hepatic failure (FHF) patient serum with the cells. We have found that FHF serum inhibits the growth rate and the synthesis of DNA, RNA, and protein; disturbs glutathione homeostasis; and induces morphological changes in cultured human Hep G2 cells. These interactions should influence the design of bioartificial liver devices based on proliferating cell lines and indicate the requirement to pretreat FHF patient plasma to reduce the toxin load.

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The effects of serum from patients with acute liver failure on the growth and metabolism of Hep G2 cells

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