The effects of serum from patients with acute liver failure on the growth and metabolism of Hep G2 cells

Q. Shi, J. D.S. Gaylor, R. Cousins, J. Plevris, P. C. Hayes, M. H. Grant*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

In many bioartificial liver systems currently being designed and evaluated for use in fulminant hepatic failure, direct contact is required between the patient's blood and the liver cells in the device. The efficacy of such devices will be influenced by the interaction of fulminant hepatic failure (FHF) patient serum with the cells. We have found that FHF serum inhibits the growth rate and the synthesis of DNA, RNA, and protein; disturbs glutathione homeostasis; and induces morphological changes in cultured human Hep G2 cells. These interactions should influence the design of bioartificial liver devices based on proliferating cell lines and indicate the requirement to pretreat FHF patient plasma to reduce the toxin load.

Original languageEnglish
Pages (from-to)1023-1030
Number of pages8
JournalArtificial organs
Volume22
Issue number12
DOIs
Publication statusPublished - 1998

Keywords / Materials (for Non-textual outputs)

  • Bioartificial liver
  • Cytotoxicity of fulminant hepatic failure serum
  • Hep G2 cells

Fingerprint

Dive into the research topics of 'The effects of serum from patients with acute liver failure on the growth and metabolism of Hep G2 cells'. Together they form a unique fingerprint.

Cite this