The Escherichia coli stpA gene is transiently expressed during growth in rich medium and is induced in minimal medium and by stress conditions

A Free, C J Dorman

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The transcriptional regulation of the stpA gene, encoding the Escherichia coli H-NS-like protein StpA, has been studied as a function of a variety of environmental conditions, and its response to trans-acting factors has been characterized. Chromosomally located stpA is expressed primarily from a promoter immediately upstream of the gene which is severely repressed by the homologous nucleoid-associated protein H-NS. However, we show here that even in a strain containing functional H-NS, stpA is transiently induced during growth of a batch culture in rich medium. It can also be induced strongly by osmotic shock and, to a lesser extent, by an increase in growth temperature. Moreover, when cells are grown in minimal medium, we observe a more sustained induction of stpA which is dependent on the leucine-responsive regulatory protein (Lrp). This enhanced level of stpA transcription is virtually abolished in an H-NS-independent manner when the culture undergoes carbon starvation. A sensitivity of the stpA promoter to DNA topology may contribute to some of these responses. Results reported here show that cloned fragments of the stpA promoter region can confer H-NS and Lrp responsiveness upon a lacZ reporter gene and suggest that several hundred base pairs of DNA upstream of the transcriptional start may be required for regulation by these two proteins.

Original languageEnglish
Pages (from-to)909-18
Number of pages10
JournalJournal of Bacteriology
Issue number3
Publication statusPublished - Feb 1997

Keywords / Materials (for Non-textual outputs)

  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • Carbon
  • Carrier Proteins
  • Chromosomes, Bacterial
  • Culture Media
  • DNA, Bacterial
  • DNA-Binding Proteins
  • Escherichia coli
  • Escherichia coli Proteins
  • Gene Expression Regulation, Bacterial
  • Genes, Bacterial
  • Hot Temperature
  • Integration Host Factors
  • Low Density Lipoprotein Receptor-Related Protein-1
  • Molecular Chaperones
  • Mutation
  • Nucleic Acid Conformation
  • Osmotic Pressure
  • Promoter Regions, Genetic
  • Receptors, Immunologic
  • Recombinant Fusion Proteins
  • Starvation
  • Suppression, Genetic
  • Time Factors


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